An unconventional two-stage cultivation strategy to increase the lipid content and enhance the fatty acid profile on Chlorella minutissima biomass cultivated in a novel internal light integrated photobioreactor aiming at biodiesel production
| Autor: | Mateus S. Amaral, Cristiano E. R. Reis, Arnaldo Márcio Ramalho Prata, Heizir F. de Castro, Fabiano Luiz Naves, Messias Borges Silva, Guilherme A. Pedro, Carla Cristina Almeida Loures |
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| Přispěvatelé: | Universidade de São Paulo (USP), Univ Fed Ouro Preto, Ctr Fed Educ Tecnol Celso Suckow Fonseca, Univ Fed Sao Joao del Rei, Universidade Estadual Paulista (Unesp) |
| Rok vydání: | 2020 |
| Předmět: |
chemistry.chemical_classification
Two-stage cultivation Biodiesel 060102 archaeology Renewable Energy Sustainability and the Environment Chemistry 020209 energy Bioreactor Photobioreactor Biomass Fatty acid 06 humanities and the arts 02 engineering and technology Raw material Palmitic acid chemistry.chemical_compound Chlorella minutissima Biodiesel production Microalgae 0202 electrical engineering electronic engineering information engineering 0601 history and archaeology Food science Cetane number |
| Zdroj: | Web of Science Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| ISSN: | 0960-1481 |
| DOI: | 10.1016/j.renene.2020.04.084 |
| Popis: | Made available in DSpace on 2020-12-10T17:34:51Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-08-01 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Biodiesel quality is dependent upon the fatty acids distribution of the raw material used in the trans/esterification reactions. While microalgae cells are regarded as potential oil suppliers for the biodiesel production chain, a common criticism is related to the low lipid productivities and often to a poor fatty acid distribution. In the present work we propose to submit microalgal cultivation to a second stage with nutritional stress by a simple addition of a 70% nitrate source depleted medium at the beginning of stationary phase, without harvesting cells, saving time and water. Chlorella minutissima was cultivated in a novel unconventional photobioreactor, named Internally Illuminated Integrated Photobioreactor (ILIPBR), to verify the efficiency of the proposed strategy. Biomass concentration, lipid content and volumetric lipids productivity increased 24.9%, 120.7% and 55.6%, respectively, from Stage 1 to Stage 2, when ILI-PBR was illuminated by constant blue LED and 0.5 vvm of aeration. The fatty acid profiles, evaluated in terms of LED illumination (white, red and blue), showed a predominance of palmitic acid in all cases (59.49%, 57.28% and 46.51%, respectively), which allowed to enhance some biodiesel properties such as cetane number, cold filter plugging point, density and kinematic viscosity, according to international specifications. (C) 2020 Elsevier Ltd. All rights reserved. Univ Sao Paulo, Engn Sch Lorena, Lorena, SP, Brazil Univ Fed Ouro Preto, Inst Exact & Biol Sci, Dept Chem, Lab Technol & Environm Chem, Ouro Preto, MG, Brazil Ctr Fed Educ Tecnol Celso Suckow Fonseca, Angra Dos Reis, RJ, Brazil Univ Fed Sao Joao del Rei, Alto Paraopeba Campus, Ouro Branco, MG, Brazil Univ Estadual Paulista, Engn Fac Guaratingueta, Guaratingueta, SP, Brazil Univ Estadual Paulista, Engn Fac Guaratingueta, Guaratingueta, SP, Brazil |
| Databáze: | OpenAIRE |
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