Preparation and biological evaluation of radiolabelled antibodies with selected carbohydrate modifications
| Autor: | P. Qi, Thomas R. Sykes, R.R. Koganty, S. Selvaraj, Antoine A. Noujaim |
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| Rok vydání: | 1993 |
| Předmět: |
Male
Cancer Research Biodistribution Molecular Sequence Data Carbohydrates Drug Evaluation Preclinical Acetylgalactosamine Models Biological Reductive amination Immunoscintigraphy Glutarates Iodine Radioisotopes Mice chemistry.chemical_compound Animals Humans Tissue Distribution Radiology Nuclear Medicine and imaging Mice Inbred ICR Immunotoxins Carbohydrate Sialic acid Carbohydrate Sequence Biochemistry chemistry Immunoglobulin G Isotope Labeling Galactose Molecular Medicine Conjugate |
| Zdroj: | Nuclear Medicine and Biology. 20:453-459 |
| ISSN: | 0969-8051 |
| Popis: | Two carbohydrates, N -acetylgalactosamine (GalNAc) and galactose-β -1,3-GalNAc have been attached to human IgG (hIgG) by a novel linking reagent, hexafluoroglutaric acid dimethyl ester. Fluorine-19 NMR signals were used for the determination of the conjugation ratio. A third carbohydrate, sialic acid, was conjugated via reductive amination and the conjugation ratio determined by a resorcinol assay. The biological behaviour of these radioiodinated antibodies with carbohydrate modification in normal mice indicates an enhanced liver uptake at 15 min post-injection with an associated change in circulating blood levels occurs for the galactose-based hIgG preparations. However, no significant differences in the biodistribution were observed for the sialic acid conjugate. These studies confirm the potential of carbohydrate-antibody conjugation for modifying the behaviour of antibodies in immunoscintigraphy and radioimmunotherapy. |
| Databáze: | OpenAIRE |
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