The origin of the diversity of crotoxin isoforms in the venom of Crotalus durissus terrificus
| Autor: | Christiane Bouchier, Grazyna Faure, Marc Vuilhorgne, Cassian Bon, Valérie Choumet, Jean-Luc Guillaume, B. Monegier, Luc Camoin |
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| Rok vydání: | 1994 |
| Předmět: |
Gene isoform
Protein subunit Molecular Sequence Data Venom Biology medicine.disease_cause complex mixtures Biochemistry Mass Spectrometry Phospholipase A2 Crotalid Venoms medicine Animals South American rattlesnake Amino Acid Sequence Sequence Homology Amino Acid Toxin cDNA library Crotalus Genetic Variation biology.organism_classification Chromatography Ion Exchange Crotoxin Molecular biology Snake venom biology.protein Chromatography Gel Electrophoresis Polyacrylamide Gel |
| Zdroj: | European journal of biochemistry. 223(1) |
| ISSN: | 0014-2956 |
| Popis: | Crotoxin, the main toxin from the venom of the South American rattlesnake Crotalus durissus terrificus, is a β-neurotoxin which consists of the non-covalent association of two subunits: a phospholipase A2 subunit B (CB), and a non-enzymic subunit A (CA). We have previously purified and characterized several isoforms of each subunit of crotoxin in the venom collected from numerous snakes. Furthermore, three cDNAs encoding two CB isoforms and the precursor, pro-CA, of subunit A have been isolated from a cDNA library prepared from a single venom gland of Crotalus durissus terrificus. The aim of this study is to analyse an individual snake venom from an animal that has been used to construct a cDNA library. Several isoforms of subunit A and two isoforms of subunit B were isolated and compared to purified and characterized subunit isoforms from pooled venom. The result of this study showed that the multiplicity and the diversity of crotoxin isoforms result from post-translational modifications occurring on a precursor and from the expression of different messenger RNAs present in an individual snake. It allowed for the identification of the two CB isoforms encoding cDNAs expressed in the individual venom with two isoforms from pooled venom, CBc and probably CBa2, that belong to two classes of crotoxin complexes which can be distinguished biochemically and pharmacologically. |
| Databáze: | OpenAIRE |
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