Development of a new ‘ultrametric’ method for assessing spawning progression in female teleost serial spawners
| Autor: | Katerina Charitonidou, Olav Sigurd Kjesbu, Thassya C. dos Santos Schmidt, Kelli Anderson, Anders Thorsen, Kostas Ganias, Grethe Thorsheim, Maud Alix |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Population dynamics Sample point Oviposition Science Zoology Fish reproduction Biology 010603 evolutionary biology 01 natural sciences Article Workflow Automation medicine Animals Ultrametric space Marine biology Multidisciplinary 010604 marine biology & hydrobiology Vitellogenesis Organ Size Oocyte biology.organism_classification Gonadosomatic Index medicine.anatomical_structure Fully automated Gadus morhua Oocytes Medicine Female Atlantic cod |
| Zdroj: | Scientific Reports, Vol 10, Iss 1, Pp 1-13 (2020) Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | The collection and presentation of accurate reproductive data from wild fish has historically been somewhat problematic, especially for serially spawning species. Therefore, the aim of the current study was to develop a novel method of assessing female spawning status that is robust to variation in oocyte dynamics between specimens. Atlantic cod (Barents Sea stock) were used to develop the new ‘ultrametric’ method, that is based on the progressive depletion of the vitellogenic oocyte pool relative to the rather constant previtellogenic oocyte (PVO) pool. Fish were subsequently partitioned into one of four categories that accurately reflected changes in their oocyte size frequency distribution characteristics and gonadosomatic index throughout spawning. The ultrametric method overcomes difficulties associated with presence of bimodal oocyte distributions, oocyte tails, lack of clear hiatus region, and presence of free ova, and can be implemented at a single sampling point. Much of the workflow is fully automated, and the technique may circumvent the need for histological analysis depending on the desired outcome. The ultrametric method differs from the traditional autodiametric method in that PVOs can be separated by ultrasonication and then enumerated, and ovarian homogeneity is not a mandatory requirement per se. The method is designed for determinate spawners but might be extended to include indeterminate spawners. |
| Databáze: | OpenAIRE |
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