Assay of acyclovir in human skin layers by high-performance liquid chromatography
| Autor: | Patrizia Santi, Paolo Colombo, Laureri Cf, Nadia Maria Volpato |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
viruses
Clinical Biochemistry Pharmaceutical Science Acyclovir Human skin High-performance liquid chromatography Analytical Chemistry Drug Discovery Humans Spectroscopy Chromatography High Pressure Liquid Transdermal Skin Reproducibility Chromatography integumentary system Chemistry Extraction (chemistry) virus diseases Reproducibility of Results Reversed-phase chromatography Iontophoresis Distilled water Calibration Spectrophotometry Ultraviolet Quantitative analysis (chemistry) |
| Zdroj: | Journal of pharmaceutical and biomedical analysis. 16(3) |
| ISSN: | 0731-7085 |
| Popis: | This paper describes an assay procedure for acyclovir quantification in human skin after in vitro transdermal transport experiments. The procedure employs warm distilled water for acyclovir (ACV) extraction and high-performance liquid chromatography (HPLC) as analytical method. The procedure has good reproducibility, sensitivity and specificity, resulting in a reliable method for biopharmaceutical studies of ACV distribution in skin tissue. The chromatographic conditions set up, using distilled water as mobile phase, makes the analytical procedure simple and easy to perform. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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