Nonconventional protease catalysis in frozen aqueous solutions
| Autor: | Hans-Dieter Jakubke, Marion Hänsler |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Proteases
medicine.medical_treatment Biochemistry Catalysis Serine chemistry.chemical_compound Structural Biology Drug Discovery Endopeptidases Freezing medicine Peptide synthesis Organic chemistry Molecular Biology Pharmacology chemistry.chemical_classification Protease Aqueous solution Organic Chemistry Water General Medicine Solutions Enzyme chemistry Molecular Medicine Peptides Cysteine |
| Zdroj: | Journal of peptide science : an official publication of the European Peptide Society. 2(5) |
| ISSN: | 1075-2617 |
| Popis: | During the past decade proteases have been widely used as catalysts in peptide synthesis. Unfortunately, they are not ideal ligases. Enzymatic peptide synthesis in frozen aqueous systems has been developed as an approach towards the suppression of competitive reactions. This paper summarizes reports concerning the behaviour of non-enzymatic as well as of enzyme-catalysed reactions when the reaction mixture is frozen. The advantages of freezing the reaction mixture in serine and cysteine protease-catalysed peptide synthesis, the influence of modified reaction conditions and the possible reasons for the yield-increasing effect of freezing are discussed. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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