Campylobacter jejuni increases the paracellular permeability of broiler chickens in a dose-dependent manner
| Autor: | Daniel Ruhnau, Wageha A. Awad, Michael Hess, Claudia Hess |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Male
Cell Membrane Permeability medicine.disease_cause Campylobacter jejuni Microbiology Jejunum 03 medical and health sciences Cecum Campylobacter Infections medicine Animals Large intestine Intestinal Mucosa Poultry Diseases lcsh:SF1-1100 030304 developmental biology broiler chickens Colony-forming unit 0303 health sciences Ussing chamber biology Campylobacter digestive oral and skin physiology 0402 animal and dairy science Broiler 04 agricultural and veterinary sciences General Medicine Immunology Health and Disease biology.organism_classification colonization 040201 dairy & animal science medicine.anatomical_structure bacterial dose Animal Science and Zoology Female lcsh:Animal culture permeability Chickens |
| Zdroj: | Poultry Science Poultry Science, Vol 99, Iss 11, Pp 5407-5414 (2020) |
| ISSN: | 1525-3171 0032-5791 |
| Popis: | In recent years, several studies emphasize the deleterious effects of Campylobacter jejuni on the chicken intestine. In this context, it was shown that C. jejuni, contrary to the general belief, has a negative influence on the gut barrier in chickens. More precisely, we demonstrated that C. jejuni affects gut physiology characterized by changes in ion transport and transepithelial ion conductance, but the underlying mechanism is yet to be investigated. In the actual study, to determine epithelial paracellular permeability, the mucosal to serosal flux of 14C-mannitol in the small and large intestine was measured applying Ussing chamber. A total of seventy-five 1-day-old Ross 308 broiler chickens were housed in floor pens on wood shavings with feed and water provided ad libitum. Birds were randomly allocated to 3 different groups (n = 25 with 5 replicates/group) and infected at 14 d of age with a high (108 colony forming units [CFU]) or a low (104 CFU) dose of C. jejuni and a third group kept as noninfected control. Infection with the low dose of C. jejuni resulted in delayed cecal colonization but equalized at 21 d postinfection, independent of the dose. Invasion of liver and spleen with C. jejuni was only noticed in birds infected with 108 (CFU). Body weight (BW) and body weight gain of all birds infected with C. jejuni were lower than in the control group and varied with the dose of infection, confirming a negative correlation between the infection dose and birds BW. Mannitol flux in jejunum and cecum was significantly (P |
| Databáze: | OpenAIRE |
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