Effect of hyaluronic acid on human chondrocyte cell lines from articular cartilage
| Autor: | Anna Maria Patti, C. Della Rocca, A. Vulcano, M.T. Ramieri, A. Gabriele |
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| Rok vydání: | 2001 |
| Předmět: |
Adult
Cartilage Articular cell lines Biology Cell Line chemistry.chemical_compound human articular cartilage Chondrocytes Adjuvants Immunologic Hyaluronic acid medicine Cell Adhesion Humans Hyaluronic Acid Aged Cartilage Cell Biology General Medicine Anatomy Adhesion Metabolism Middle Aged Molecular biology In vitro medicine.anatomical_structure chemistry chondrocytes hyaluronic acid Cell culture Collagenase Digestion Energy Metabolism Cell Division Developmental Biology medicine.drug |
| Zdroj: | Tissuecell. 33(3) |
| ISSN: | 0040-8166 |
| Popis: | The effects of hyaluronic acid (HA) derivative on the proliferation and metabolism of human chondrocytes were examined. Cells were obtained from cartilage from metatarsal phalangeal joints of 20 adult humans (aged 22-63) and from femoral knee condyles of 10 subjects (aged 22-77). Chondrocytes isolated by collagenase/Dnase digestion were cultured with addition of different doses of HA for 4 weeks. Morphological studies demonstrated that HA enhanced the adhesion of cells to substrate; HA-treated chondrocytes proliferated better than chondrocytes cultured in HA-free medium. This study shows that HA improves in vitro substrate adhesion ability and proliferative activity of human cartilage cells and that the response to the treatment varies on an individual basis. |
| Databáze: | OpenAIRE |
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