A capacitive biosensor based on an interdigitated electrode with nanoislands
| Autor: | Ha Wook Jung, Jae Chul Pyun, Sungbo Cho, Young Wook Chang, Min Jung Kang, Ga Yeon Lee |
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| Rok vydání: | 2014 |
| Předmět: |
Immunoassay
Analyte biology Chemistry Model protein Nanotechnology Biosensing Techniques Enzymes Immobilized Biochemistry Horseradish peroxidase Capacitive detection Analytical Chemistry Nanostructures Electrode biology.protein Interdigitated electrode Environmental Chemistry Capacitive biosensor Electrodes Spectroscopy Horseradish Peroxidase Protein adsorption |
| Zdroj: | Analytica chimica acta. 844 |
| ISSN: | 1873-4324 |
| Popis: | A capacitive biosensor based on an interdigitated electrode (IDE) with nanoislands was developed for label-free detection of antigen-antibody interactions. To enable sensitive capacitive detection of protein adsorption, the nanoislands were fabricated between finger electrodes of the IDE. The effect of the nanoislands on the sensitive capacitive measurement was estimated using horseradish peroxidase (HRP) as a model protein. Additionally, a parylene-A film was coated on the IDE with nanoislands to improve the efficiency of protein immobilization. By using HRP and hepatitis B virus surface antigen (HBsAg) as model analytes, the effect of the parylene-A film on the capacitive detection of protein adsorption was demonstrated. |
| Databáze: | OpenAIRE |
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