Quantifying Memory CD8 T Cells Reveals Regionalization of Immunosurveillance
| Autor: | Jason M. Schenkel, Lalit K. Beura, Luke S. Manlove, David Masopust, Kathryn A. Fraser, Botond Z. Igyártó, Peter J. Southern, Elizabeth M. Steinert |
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| Rok vydání: | 2014 |
| Předmět: |
Antigens
Differentiation T-Lymphocyte Lymphocyte Population Biology CD8-Positive T-Lymphocytes General Biochemistry Genetics and Molecular Biology Article Mice Antigen Memory cell Antigens CD Cell Movement Monitoring Immunologic T-Lymphocyte Subsets medicine Cytotoxic T cell Animals Arenaviridae Infections Lymphocytic choriomeningitis virus Lectins C-Type education Inflammation education.field_of_study Biochemistry Genetics and Molecular Biology(all) Intracellular parasite Cell biology Immunosurveillance Mice Inbred C57BL medicine.anatomical_structure Immunology Immunologic Memory Homing (hematopoietic) |
| Zdroj: | Cell. 161(4) |
| ISSN: | 1097-4172 |
| Popis: | SummaryMemory CD8 T cells protect against intracellular pathogens by scanning host cell surfaces; thus, infection detection rates depend on memory cell number and distribution. Population analyses rely on cell isolation from whole organs, and interpretation is predicated on presumptions of near complete cell recovery. Paradigmatically, memory is parsed into central, effector, and resident subsets, ostensibly defined by immunosurveillance patterns but in practice identified by phenotypic markers. Because isolation methods ultimately inform models of memory T cell differentiation, protection, and vaccine translation, we tested their validity via parabiosis and quantitative immunofluorescence microscopy of a mouse memory CD8 T cell population. We report three major findings: lymphocyte isolation fails to recover most cells and biases against certain subsets, residents greatly outnumber recirculating cells within non-lymphoid tissues, and memory subset homing to inflammation does not conform to previously hypothesized migration patterns. These results indicate that most host cells are surveyed for reinfection by segregated residents rather than by recirculating cells that migrate throughout the blood and body. |
| Databáze: | OpenAIRE |
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