An Induction in Hepatic HDL Secretion Associated with Reduced ATPase Expression
| Autor: | Nihar R. Pandey, Ayesha Misquith, Erin Twomey, Daniel L. Sparks, Lara Kouri, Joanna Renwick, Seham Rabaa |
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| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
medicine.medical_specialty
ATPase Cell Endocytic recycling ATP-binding cassette transporter Stimulation Biology Models Biological Pathology and Forensic Medicine Linoleic Acid Leucine Internal medicine Glyburide medicine Humans Secretion RNA Small Interfering Phospholipids ATP Binding Cassette Transporter Subfamily G Member 1 Liver X Receptors Clofibrate Apolipoprotein A-I nutritional and metabolic diseases Hep G2 Cells Orphan Nuclear Receptors Culture Media Proton-Translocating ATPases medicine.anatomical_structure Endocrinology Liver biology.protein lipids (amino acids peptides and proteins) ATP-Binding Cassette Transporters Lipoproteins HDL Protein Processing Post-Translational Lipoprotein medicine.drug Regular Articles ATP Binding Cassette Transporter 1 |
| Popis: | Linoleic acid-phospholipids stimulate high-density lipoprotein (HDL) net secretion from liver cells by blocking the endocytic recycling of apoA-I. Experiments were undertaken to determine whether apoA-I accumulation in the cell media is associated with membrane ATPase expression. Treatment of HepG2 cells with dilinoeoylphosphatidylcholine (DLPC) increased apoA-I secretion fourfold. DLPC also significantly reduced cell surface F1-ATPase expression and reduced cellular ATP binding cassette (ABC)A1 and ABCG1 protein levels by approximately 50%. In addition, treatment of HepG2 cells with the ABC transporter inhibitor, glyburide, stimulated the apoA-I secretory effects of both DLPC and clofibrate. Pretreatment of HepG2 cells with compounds that increased ABC transport protein levels (TO901317, N-Acetyl-L-leucyl-L-leucyl-L-norleucinal, and resveratrol) blocked the DLPC-induced stimulation in apoA-I net secretion. Furthermore, whereas HepG2 cells normally secrete nascent prebeta-HDL, DLPC treatment promoted secretion of alpha-migrating HDL particles. These data show that an linoleic acid-phospholipid induced stimulation in hepatic HDL secretion is related to the expression and function of membrane ATP metabolizing proteins. |
| Databáze: | OpenAIRE |
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