The localization of the different forms of troponin I in skeletal and cardiac muscle cells
| Autor: | S. Victor Perry, P. G. H. Gell, Gurtej K. Dhoot |
|---|---|
| Rok vydání: | 1978 |
| Předmět: |
Immunodiffusion
ATPase Muscle Proteins Hamster Biology Antibodies Immunoenzyme Techniques Species Specificity Cricetinae Troponin I medicine Animals Humans Muscle fibre Adenosine Triphosphatases Muscles Myocardium Cardiac muscle Skeletal muscle Haplorhini Cell Biology Macaca mulatta Molecular biology Troponin Rats Staining medicine.anatomical_structure Biochemistry biology.protein Rabbits Antibody |
| Zdroj: | Experimental Cell Research. 117:357-370 |
| ISSN: | 0014-4827 |
| DOI: | 10.1016/0014-4827(78)90149-0 |
| Popis: | 1. 1. Antibodies raised against troponin I isolated from human cardiac and rabbit fast and slow skeletal muscles have been shown to be specific for the polymorphic forms of troponin I against which they were raised, i.e. they are tissue specific. 2. 2. These antibodies reacted with the polymorphic forms of troponin I, against which they were raised, that are present in tissues of other species such as the rhesus monkey, hamster and rat, i.e. they were species non-specific. 3. 3. Using the immunoperoxidase staining technique it has been shown that the fast and slow forms of troponin I are located in different cells in virtually all adult normal muscles examined. 4. 4. By comparison of the ATPase staining of skeletal muscle sections at pH 9.4 and 4.2 it is concluded that the fast form of troponin I is located in type II fibres and the slow form in type I fibres. 5. 5. It is suggested that immunoperoxidase staining with the antibodies to the fast and slow forms of troponin I provides an unambiguous new method of muscle fibre typing. |
| Databáze: | OpenAIRE |
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