The Triplo-lethal locus of Drosophila: reexamination of mutants and discovery of a second-site suppressor
| Autor: | Douglas R. Dorer, Alan C. Christensen, D H Ezekiel |
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| Rok vydání: | 1995 |
| Předmět: |
Genetic Linkage
Mutant Molecular Sequence Data Locus (genetics) Genes Insect Genes Recessive Investigations Genome Genetic linkage Gene duplication Genetics Animals Allele Genes Suppressor Recombination Genetic biology Base Sequence Genetic Complementation Test Chromosome Mapping Gene Expression Regulation Developmental biology.organism_classification Molecular biology Drosophila melanogaster Mutagenesis DNA Transposable Elements Genes Lethal Ploidy Gene Deletion |
| Zdroj: | Genetics. 141(3) |
| ISSN: | 0016-6731 |
| Popis: | In the genome of Drosophila melanogaster there is a single locus, Triplo-lethal (Tpl), that causes lethality when present in either one or three copies in an otherwise diploid animal. Previous attempts to mutagenize Tpl produced alleles that were viable over a chromosome bearing a duplication of Tpl, but were not lethal in combination with a wild-type chromosome, as deficiencies for Tpl are. These mutations were interpreted as hypomorphic alleles of Tpl. In this work, we show that these alleles are not mutations at Tpl; rather, they are dominant mutations in a tightly linked, but cytologically distant, locus that we have named Suppressor-of-Tpl (Sul(Tpl)). Su(Tpl) mutations suppress the lethality associated with three copies of the Triplo-lethal locus and are recessive lethal. We have mapped Su(Tpl) to the approximate map position 3-46.5, within the cytological region 76B-76D. |
| Databáze: | OpenAIRE |
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