Investigating Tunneling Nanotubes in Cancer Cells: Guidelines for Structural and Functional Studies through Cell Imaging

Autor: Baptiste Monterroso, Damien Schapman, Céline Bazille, Magalie Bénard, Hélène Roberge, Alexis Lebon, Emmanuel Bergot, Guénaëlle Levallet, Jérôme Levallet, Hitoshi Komuro, Fatéméh Dubois, Ludovic Galas, Bastien Jean-Jacques, Nicolas Elie, Didier Goux
Přispěvatelé: Imagerie et Stratégies Thérapeutiques des pathologies Cérébrales et Tumorales (ISTCT), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS), Département de Pathologie [CHU Caen], Normandie Université (NU)-Normandie Université (NU)-CHU Caen, Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Tumorothèque de Caen Basse-Normandie (TCBN), Plate-Forme de Recherche en Imagerie Cellulaire de Haute-Normandie (PRIMACEN), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Institute for Research and Innovation in Biomedicine (IRIB), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Interactions Cellules Organismes Environnement (ICORE), CHU Caen, Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Université de Caen Normandie (UNICAEN), Normandie Université (NU), Yale University [New Haven], Service de pneumologie [CHU Caen], Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-High-tech Research Infrastructures for Life Sciences (HeRacLeS), Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0301 basic medicine
Electron Microscope Tomography
Stromal cell
[SDV]Life Sciences [q-bio]
Cell
[SDV.CAN]Life Sciences [q-bio]/Cancer
Review Article
Cell Communication
medicine.disease_cause
Microtubules
PC12 Cells
[SDV.MHEP.PSR]Life Sciences [q-bio]/Human health and pathology/Pulmonology and respiratory tract
General Biochemistry
Genetics and Molecular Biology
03 medical and health sciences
0302 clinical medicine
In vivo
Neoplasms
medicine
Animals
Humans
Cytoskeleton
General Immunology and Microbiology
STED microscopy
Cancer
General Medicine
medicine.disease
3. Good health
Rats
030104 developmental biology
medicine.anatomical_structure
030220 oncology & carcinogenesis
Cancer cell
Biophysics
Microscopy
Electron
Scanning
Medicine
Carcinogenesis
[SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Zdroj: BioMed Research International
BioMed Research International, Hindawi Publishing Corporation, 2020, 2020, pp.1-16. ⟨10.1155/2020/2701345⟩
BioMed Research International, Vol 2020 (2020)
BioMed Research International, 2020, 2020, pp.1-16. ⟨10.1155/2020/2701345⟩
ISSN: 2314-6133
2314-6141
DOI: 10.1155/2020/2701345⟩
Popis: By allowing insured communication between cancer cells themselves and with the neighboring stromal cells, tunneling nanotubes (TNTs) are involved in the multistep process of cancer development from tumorigenesis to the treatment resistance. However, despite their critical role in the biology of cancer, the study of the TNTs has been announced challenging due to not only the absence of a specific biomarker but also the fragile and transitory nature of their structure and the fact that they are hovering freely above the substratum. Here, we proposed to review guidelines to follow for studying the structure and functionality of TNTs in tumoral neuroendocrine cells (PC12) and nontumorigenic human bronchial epithelial cells (HBEC-3, H28). In particular, we reported how crucial is it (i) to consider the culture conditions (culture surface, cell density), (ii) to visualize the formation of TNTs in living cells (mechanisms of formation, 3D representation), and (iii) to identify the cytoskeleton components and the associated elements (categories, origin, tip, and formation/transport) in the TNTs. We also focused on the input of high-resolution cell imaging approaches including Stimulated Emission Depletion (STED) nanoscopy, Transmitted and Scanning Electron Microscopies (TEM and SEM). In addition, we underlined the important role of the organelles in the mechanisms of TNT formation and transfer between the cancer cells. Finally, new biological models for the identification of the TNTs between cancer cells and stromal cells (liquid air interface, ex vivo, in vivo) and the clinical considerations will also be discussed.
Databáze: OpenAIRE
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