A 680 kb duplication at the FTO locus in a kindred with obesity and a distinct body fat distribution
| Autor: | Thet Naing, Ruth McPherson, Paulina Lau, Majid Nikpay, Robert Dent, Robert W. Davies, Heather Doelle, Mary-Ellen Harper |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Adult
Male DNA Copy Number Variations Genotype Alpha-Ketoglutarate-Dependent Dioxygenase FTO Locus (genetics) Genome-wide association study Biology FTO gene Polymorphism Single Nucleotide Article White People Body Mass Index Gene duplication Genetics medicine Humans Genetic Predisposition to Disease Copy-number variation Obesity Genetics (clinical) nutritional and metabolic diseases Proteins Middle Aged medicine.disease Adipose Tissue RPGRIP1L Female Genome-Wide Association Study |
| Popis: | Common intronic SNPs in the human fat mass and obesity associated (FTO) gene are strongly associated with body mass index (BMI). In mouse models, inactivation of the Fto gene results in a lean phenotype, whereas overexpression of Fto leads to increased food intake and obesity. The latter finding suggests that copy number variants at the FTO locus might be associated with extremes of adiposity. To address this question, we searched for rare, private or de novo copy number variation in a cohort of 985 obese and 869 lean subjects of European ancestry drawn from the extremes of the BMI distribution, genotyped on Affymetrix 6.0 arrays. A ∼680 kb duplication, confirmed by real-time PCR and G-to-FISH analyses, was observed between ∼rs11859825 and rs9932411 in a 68-year-old male with severe obesity. The duplicated region on chromosome 16 spans the entire genome-wide association studies risk locus for obesity, and further encompasses RBL2, AKTIP, RPGRIP1L and all but the last exon of the FTO gene. Affected family members exhibit a unique obesity phenotype, characterized by increased fat distribution in the shoulders and neck with a significantly increased neck circumference. This phenotype was accompanied by increased peripheral blood expression of RBL2 with no alteration in expression of FTO or other genes in the region. No other duplications or deletions in this region were identified in the cohort of obese and lean individuals or in a further survey of 4778 individuals, suggesting that large rare copy number variants surrounding the FTO gene are not a frequent cause of obesity. |
| Databáze: | OpenAIRE |
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