Maintaining multipotent trunk neural crest stem cells as self-renewing crestospheres
| Autor: | Aldo Castillo, Christina Murko, Camilla Persson, Sofie Mohlin, Ezgi Kunttas, Marianne E. Bronner, Reem Abdel-Haq, Laura Kerosuo |
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| Přispěvatelé: | Department of Biochemistry and Developmental Biology, Medicum |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
EXPRESSION
Chick Embryo Biology Fibroblast growth factor Article CULTURE 03 medical and health sciences 0302 clinical medicine Cranial neural crest Neural Stem Cells Crestospheres RETINOIC ACID medicine Animals FGF Stem cell maintenance Progenitor cell Molecular Biology 030304 developmental biology Progenitor 0303 health sciences ROLES Multipotent Stem Cells Neural tube 1184 Genetics developmental biology physiology Neural crest Cell Differentiation Cell Biology Trunk Multipotency self-renewal medicine.anatomical_structure DIFFERENTIATION Neural Crest embryonic structures 1182 Biochemistry cell and molecular biology Stem cell Neural crest stem cells Chickens Neuroscience 030217 neurology & neurosurgery Developmental Biology Trunk neural crest |
| Popis: | Neural crest cells have broad migratory and differentiative ability that differs according to their axial level of origin. However, their transient nature has limited understanding of their stem cell and self-renewal properties. While an in vitro culture method has made it possible to maintain cranial neural crest cells as self-renewing multipotent crestospheres (Kerosuo et al., 2015), these same conditions failed to preserve trunk neural crest in a stem-like state. Here we optimize culture conditions for maintenance of avian trunk crestospheres, comprised of both neural crest stem and progenitor cells. Our trunk-derived crestospheres are multipotent and display self-renewal capacity over several weeks. Trunk crestospheres display elevated expression of neural crest cell markers as compared to those characteristic of ventrolateral neural tube or mesodermal fates. Moreover, trunk crestospheres express increased levels of trunk neural crest-enriched markers as compared to cranial crestospheres. Finally, we use lentiviral transduction as a tool to manipulate gene expression in trunk crestospheres. Taken together, this method enables long-term in vitro maintenance and manipulation of multipotent trunk neural crest cells in a premigratory stem or early progenitor state. Trunk crestospheres are a valuable resource for probing mechanisms underlying neural crest sternness and lineage decisions as well as accompanying diseases. |
| Databáze: | OpenAIRE |
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