Iloprost supports early development of in vitro-produced porcine embryos through activation of the phosphatidylinositol 3-kinase/AKT signalling pathway

Autor: Kyu-Tae Chang, Seon-A Choi, Hae-Jun Yang, Bong-Seok Song, Young-Ho Park, Young Il Park, Jae-Won Huh, Sang-Rae Lee, Deog-Bon Koo, Youngjeon Lee, Seung-Bin Yoon, Pil-Soo Jeong, Bo-Woong Sim, Yeung Bae Jin, Ji-Su Kim, Philyong Kang, Sun-Uk Kim, Young-Hyun Kim, Seong-Eun Mun, Kang Jin Jeong
Rok vydání: 2017
Předmět:
0301 basic medicine
Nuclear Transfer Techniques
medicine.medical_specialty
animal structures
Parthenogenesis
Sus scrofa
Embryonic Development
Fertilization in Vitro
Reproductive technology
Biology
Models
Biological
Embryo Culture Techniques
Andrology
Wortmannin
Phosphatidylinositol 3-Kinases
03 medical and health sciences
chemistry.chemical_compound
Endocrinology
Internal medicine
Genetics
medicine
Animals
Inner cell mass
Iloprost
Blastocyst
Molecular Biology
PI3K/AKT/mTOR pathway
Embryo culture
Epoprostenol
Culture Media
Androstadienes
030104 developmental biology
medicine.anatomical_structure
Reproductive Medicine
chemistry
embryonic structures
cardiovascular system
Somatic cell nuclear transfer
Female
lipids (amino acids
peptides
and proteins)
Animal Science and Zoology
Proto-Oncogene Proteins c-akt
Signal Transduction
Developmental Biology
Biotechnology
medicine.drug
Zdroj: Reproduction, Fertility and Development. 29:1306
ISSN: 1031-3613
Popis: Despite evidence of the presence of prostaglandin (PG) I2 in mammalian oviducts, its role in early development of in vitro-produced (IVP) embryos is largely unknown. Thus, in the present study we examined the effects of iloprost, a PGI2 analogue, on the in vitro developmental competence of early porcine embryos and the underlying mechanism(s). To examine the effects of iloprost on the development rate of IVF embryos, iloprost was added to the in vitro culture (IVC) medium and cultured for 6 days. Supplementation of the IVC medium with iloprost significantly improved developmental parameters, such as blastocyst formation rate, the trophectoderm : inner cell mass ratio and cell survival in IVF and parthenogenetically activated (PA) embryos. In addition, post-blastulation development into the expanded blastocyst stage was improved in iloprost-treated groups compared with controls. Interestingly, the phosphatidylinositol 3-kinase (PI3K)/AKT signalling pathway was significantly activated by iloprost supplementation in a concentration-dependent manner (10–1000 nM), and the beneficial effects of iloprost on the early development of porcine IVF and PA embryos was completely ablated by treatment with 2.5 μM wortmannin, a PI3K/AKT signalling inhibitor. Importantly, expression of the PI3K/AKT signalling pathway was significantly reduced in somatic cell nuclear transfer (SCNT) compared with IVF embryos, and iloprost supported the early development of SCNT embryos, as was the case for IVF and PA embryos, suggesting a consistent effect of iloprost on the IVC of IVP porcine embryos. Together, these results indicate that iloprost can be a useful IVC supplement for production of IVP early porcine embryos with high developmental competence.
Databáze: OpenAIRE
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