Characterisation of colony-stimulating activity in the avian T cell-derived factor, Salmonella enteritidis-immune lymphokine
| Autor: | Billy M. Hargis, Edward D. McGruder, John R. DeLoach, Donald E. Corrier, Michael H. Kogut |
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| Rok vydání: | 1996 |
| Předmět: |
Salmonella enteritidis
T cell T-Lymphocytes Bone Marrow Cells Microbiology Colony-Forming Units Assay Immune system medicine Animals Trypsin Cells Cultured Poultry Diseases Lymphokines Salmonella Infections Animal General Veterinary biology Lymphokine Hematopoietic Stem Cells In vitro medicine.anatomical_structure Concanavalin A embryonic structures biology.protein Bone marrow Chickens medicine.drug |
| Zdroj: | Research in veterinary science. 60(3) |
| ISSN: | 0034-5288 |
| Popis: | This investigation was designed to characterise the specific cytokine activity from the conditioned medium of concanavalin A-stimulated avian T cells derived from Salmonella enteritidis -immune chickens, S enteritidis -immune lymphokine ( ILK ). Studies were designed to determine first, whether colony-stimulating activity was present in ILK , second, the type(s) of colonies from the bone marrow that were supported in vitro by the potential colony-stimulating factors in ILK and, third, whether colony-stimulating activity was present in serum from chicks treated with ILK and challenged with S enteritidis , and to use physicochemical treatment as a means of identifying the potential colony-stimulating factor(s) in ILK . Both ILK alone and serum from chicks treated with ILK and challenged with S enteritidis caused significant increases in the number of colony-forming units (CFU) from the bone marrow in vitro. After 10 days of incubation, ILK alone supported the in vitro growth of granulocytic bone marrow colonies. The colony-stimulating activity from serum derived from chicks treated with ILK and challenged with S enteritidis peaked two hours after the challenge. When ILK was either heated at 100°C or treated with trypsin or acid and then injected into chicks, all the chicks responded with significant increases in circulating polymorphonuclear leucocytes ( PMNS ). However, when assayed for in vitro colony-stimulating activity, only trypsinisation destroyed the activity in ILK . The results indicate that a colony-stimulating factor which preferentially supported the growth of granulocytic bone marrow colonies was present in ILK and that the factor was stable to heat and acid but sensitive to trypsin. |
| Databáze: | OpenAIRE |
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