ICPMS analysis of proteins separated by Native-PAGE: Evaluation of metaloprotein profiles in human synovial fluid with acute and chronic arthritis
| Autor: | Raul Andres Gil, Mariano Acosta, Leonardo Mariño-Repizo, Héctor Tamashiro, Mario Franco Moyano, Liliana Beatriz Villegas |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Native page Mass spectrometry 01 natural sciences Biochemistry Peroxide HUMAN SYNOVIAL FLUID PROTEINS Mass Spectrometry Inorganic Chemistry Arthritis Rheumatoid 03 medical and health sciences chemistry.chemical_compound Nitric acid Metalloproteins Synovial Fluid Metalloprotein Synovial fluid Humans Polyacrylamide gel electrophoresis Inductively coupled plasma mass spectrometry TRACE ELEMENTS chemistry.chemical_classification Chromatography Otras Ciencias Químicas 010401 analytical chemistry Ciencias Químicas 0104 chemical sciences INDUCTIVELY COUPLED PLASMA MASS SPECTROMETRY 030104 developmental biology chemistry Acute Disease Chronic Disease Molecular Medicine Electrophoresis Polyacrylamide Gel POLYACRLYLAMIDE GEL ELECTROPHORESIS CIENCIAS NATURALES Y EXACTAS |
| Zdroj: | Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS). 36 |
| ISSN: | 1878-3252 |
| Popis: | The role of trace elements bound to proteins in the etiology and pathogenesis of rheumatoid arthritis (RA) remains unclear. In this sense, the identification and detection of metalloproteins has a strong and growing interest. Metalloprotein studies are currently carried out by polyacrylamide gel electrophoresis (PAGE) associated to inductively coupled plasma mass spectrometry (ICPMS), and despite that complete information can be obtained for metals such as Fe, Cu and Zn, difficulties due to poor sensitivity for other trace elements such as Sn, As, etc, are currently faced. In the present work, a simple and fast method for the determination of trace metals bound to synovial fluid (SF) proteins was optimized. Proteins from SF (long and short-term RA) were separated in ten fractions by native PAGE, then dissolved in nitric acid and peroxide hydrogen, and analyzed by ICPMS. Fifteen metals were determined in each separated protein fraction (band). Adequate calibration of proteins molecular weight allowed stablishing which protein type were bound to different metals. Fil: Moyano, Mario Franco. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina Fil: Marino Repizo, Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina Fil: Tamashiro, Héctor. Clínica Bolivar; Argentina Fil: Villegas, Liliana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina Fil: Acosta, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina Fil: Gil, Raul Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina |
| Databáze: | OpenAIRE |
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