A novel approach to construct a horseradish peroxidase|hydrophilic ionic liquids|Au nanoparticles dotted titanate nanotubes biosensor for amperometric sensing of hydrogen peroxide
| Autor: | Yanbing Wang, Xiaoqiang Liu, Xiuhua Liu, Ruoxia Zhao, Heqing Feng |
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| Rok vydání: | 2012 |
| Předmět: |
Materials science
Conductometry Inorganic chemistry Biomedical Engineering Biophysics Ionic Liquids Infrared spectroscopy Biosensing Techniques Horseradish peroxidase chemistry.chemical_compound Nafion Electrochemistry Horseradish Peroxidase Titanium biology Nanotubes Carbon Equipment Design Hydrogen Peroxide General Medicine Chronoamperometry Amperometry Equipment Failure Analysis chemistry Ionic liquid biology.protein Gold Cyclic voltammetry Hydrophobic and Hydrophilic Interactions Biosensor Biotechnology Nuclear chemistry |
| Zdroj: | Biosensors and Bioelectronics. 31:101-104 |
| ISSN: | 0956-5663 |
| DOI: | 10.1016/j.bios.2011.09.045 |
| Popis: | The direct electrochemistry of horseradish peroxidase (HRP) on a novel sensing platform modified glassy carbon electrode (GCE) has been achieved. This sensing platform consists of Nafion, hydrophilic room-temperature ionic liquid (RTIL) and Au nanoparticles dotted titanate nanotubes (GNPs-TNTs). The composite of RTIL and GNPs-TNTs was immobilized on the electrode surface through the gelation of a small amount of HRP aqueous solution. The composite was characterized by transmission electron microscopy (TEM), powder X-ray diffraction (XRD) and infrared spectroscopy (IR). UV-Vis and IR spectroscopy demonstrated that HRP in the composite could retain its native secondary structure and biochemical activity. The HRP-immobilized electrode was investigated by cyclic voltammetry and chronoamperometry. The results from both techniques showed that the direct electron transfer between the nanocomposite modified electrodes and heme in HRP could be realized. The biosensor responded to H(2)O(2) in the linear range from 5×10(-6) to 1×10(-3) mol L(-1) with a detection limit of 2.1×10(-6) mol L(-1) (based on the S/N=3). |
| Databáze: | OpenAIRE |
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