The extract from Nandina domestica THUNBERG inhibits histamine- and serotonin-induced contraction in isolated guinea pig trachea
| Autor: | Muneo Tsukiyama, Takuro Ueki, Kazuho Abe, Tatsuhiro Akaishi, Hidenobu Okumura |
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| Rok vydání: | 2007 |
| Předmět: |
Male
Serotonin Contraction (grammar) Aporphines Guinea Pigs Pharmaceutical Science Pharmacology Biology Guinea pig chemistry.chemical_compound medicine Animals Receptors Histamine H1 Respiratory system Dose-Response Relationship Drug Plant Extracts Nantenine Muscle Smooth General Medicine Smooth muscle contraction Anatomy Berberidaceae Trachea Atropine chemistry Serotonin 5-HT2 Receptor Antagonists Histamine Acetylcholine medicine.drug Muscle Contraction |
| Zdroj: | Biologicalpharmaceutical bulletin. 30(11) |
| ISSN: | 0918-6158 |
| Popis: | Although the fruit of Nandina domestica THUNBERG (ND) has been used to treat respiratory disorders such as coughing and breathing difficulty in Japan for many years, very little is known about mechanisms underlying its action. In the present study, we investigated effects of the crude extract from ND (NDE) and one of its constituents, nantenine, on contractile responses in isolated guinea pig tracheal ring preparations. In normal experimental condition, guinea pig trachea remained tonically contracted during the resting state, and addition of NDE (1 mg/ml) caused a relaxation of tracheal smooth muscles, but had little effect on the responsiveness of trachea to acetylcholine. The basal, tonic contraction was abolished by the presence of atropine and indomethacin. In this condition, NDE at 0.1-1 mg/ml inhibited histamine-induced contraction in both competitive and non-competitive manners. NDE at 0.01-1 mg/ml inhibited serotonin-induced contraction in a competitive manner. Nantenine (2-20 microM) did not affect histamine-induced contraction, and slightly inhibited serotonin-induced contraction. These results suggest that NDE has inhibitory effects on tracheal smooth muscle contraction, and nantenine cannot account solely for this effect of NDE. |
| Databáze: | OpenAIRE |
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