Remnant lipoprotein size distribution profiling via dynamic light scattering analysis
| Autor: | Samee A. Hameed, Mia N. Nguyen, Kyana Garza, Richa Chandra, Birgit Mellis, Chandra K. Mittal, Ana V. Hernandez, James M. Jurica |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Atherogenic diet Human blood Remnant Lipoprotein Molecular Structure Chemistry Lipoproteins Biochemistry (medical) Clinical Biochemistry General Medicine 030204 cardiovascular system & hematology Biochemistry Dynamic Light Scattering 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Dynamic light scattering Humans Particle Size Size determination Lipoprotein |
| Zdroj: | Clinica chimica acta; international journal of clinical chemistry. 462 |
| ISSN: | 1873-3492 |
| Popis: | Background Remnant lipoproteins (RLP) are a metabolically derived subpopulation of triglyceride-rich lipoproteins (TRL) in human blood that are involved in the metabolism of dietary fats or triglycerides. RLP, the smaller and denser variants of TRL particles, are strongly correlated with cardiovascular disease (CVD) and were listed as an emerging atherogenic risk factor by the AHA in 2001. Methods Varying analytical techniques used in clinical studies in the size determination of RLP contribute to conflicting hypotheses in regard to whether larger or smaller RLP particles contribute to CVD progression, though multiple pathways may exist. Results We demonstrated a unique combinatorial bioanalytical approach involving the preparative immunoseparation of RLP, and dynamic light scattering for size distribution analysis. Conclusions This is a new facile and robust methodology for the size distribution analysis of RLP that in conjunction with clinical studies may reveal the mechanisms by which RLP cause CVD progression. |
| Databáze: | OpenAIRE |
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