Preliminary studies on the antibacterial activity of crude extracts and alkaloids from species ofAspidosperma

Autor: Eliana Harue Endo, Arildo José Braz de Oliveira, Francisco de A.M. Reis, Benedito Prado Dias Filho, Silvia Y. Eguchi, Celso Vataru Nakamura, Luzia Koike
Rok vydání: 2009
Předmět:
Zdroj: Pharmaceutical Biology. 47:1085-1089
ISSN: 1744-5116
1388-0209
Popis: Screening tests of hydroethanolic crude extracts of six species of Aspidosperma (Apocynaceae) against Staphylococcus aureus, Escherichia coli, Bacillus subtilis, and Pseudomonas aeruginosa were performed. Aspidosperma ramiflorum Muell. Arg. showed good activity against Bacillus subtilis with MIC and MBC of 15.7 and 125 µg/mL, moderate activity against Staphylococcus aureus with MIC and MBC of 250 and 500 µg/ mL, and weak activity against Escherichia coli with MIC and MBC of 1000 µg/mL. Aspidosperma pyricolum Muell. Arg. (MIC/MBC 125/250 µg/mL) and Aspidosperma olivaceum Muell. Arg. (MIC/MBC 250/ > 1000 µg/ mL) displayed moderate antibacterial activity against Bacillus subtilis. Separation of the crude extract of Aspidosperma ramiflorum was performed according to the usual acid–base process, which produces alkaloid mixtures and closely related metabolites. The basic fraction was active against Bacillus subtilis, Staphylococcus aureus, and Escherichia coli, with MICs of 31.2, 62.5, and 250 µg/mL, respectively. The basic fractions were more active than the acid fractions, probably because they contained some active alkaloids and/or closely related metabolites absent from the other fractions, or they contained a higher concentration of these active compounds.
Databáze: OpenAIRE
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