Pyrroloquinoxaline hydrazones as fluorescent probes for amyloid fibrils
| Autor: | Gianluca Giorgi, Sandra Gemma, O Taglialatela-Scafati, Bhupendra Prasad Joshi, Silvio Caccia, Giuseppe Campiani, Mario Salmona, Claudia Fracasso, Ettore Novellino, Isabella Fiorini, Stefania Butini, Gianluigi Forloni, Margherita Brindisi, Luisa Savini, Laura Colombo, Pierangela Tripaldi |
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| Přispěvatelé: | Gemma, S., Colombo, L., Forloni, G., Savini, L., Fracasso, C., Caccia, S., Salmona, M., Brindisi, M., Yoshi, B. P., Tripaldi, P., Giorgi, G., TAGLIALATELA SCAFATI, Orazio, Novellino, Ettore, Fiorini, I., Campiani, G., Butini, S. |
| Rok vydání: | 2011 |
| Předmět: |
Male
Models Molecular Amyloid Mice Transgenic Fibril Crystallography X-Ray Biochemistry law.invention chemistry.chemical_compound Mice Quinoxaline law In vivo Quinoxalines Animals Tissue Distribution Physical and Theoretical Chemistry Fluorescent Dyes Amyloid beta-Peptides Molecular Structure Organic Chemistry Hydrazones Brain Stereoisomerism Amyloid fibril Fluorescence In vitro Peptide Fragments Spectrometry Fluorescence chemistry Electron microscope |
| Zdroj: | Organicbiomolecular chemistry. 9(14) |
| ISSN: | 1477-0539 |
| Popis: | Here we describe the identification and preliminary characterization of a new class of pyrrolo(imidazo)quinoxaline hydrazones as flurescent probes for Aβ 1-42 fibrils. All the newly developed compounds were able to bind amyloid fibrils formed in vitro and some of them displayed an increase of their fluorescence upon binding. When tested on brain tissue preparations presenting Aβ deposits, the described hydrazones selectively stained amyloid structures and did not display aspecific binding. The hydrazones did not show antifibrillogenic activity and electron microscopy analysis revealed that they do not interfere with fibrils structure. The described pyrrolo(imidazo) quinoxalines could be useful for studying amyloid structures in vitro. Moreover, their experimentally proven ability to cross the blood-brain barrier in mouse opens the possibility of developing these compounds as potential amyloid imaging agents for in vivo applications. |
| Databáze: | OpenAIRE |
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