Transcutaneous immunotherapy via laser‐generated micropores efficiently alleviates allergic asthma in P hl p 5–sensitized mice
| Autor: | Richard Weiss, Wolf Dietrich Krautgartner, Cornelia Hauser-Kronberger, S. Kitzmueller, J. Thalhamer, Sandra Scheiblhofer, Christof Boehler, D. Bach, Esther E. Weinberger, Michael Hessenberger |
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| Rok vydání: | 2012 |
| Předmět: |
Allergy
Ovalbumin medicine.medical_treatment Molecular Sequence Data Immunology Administration Cutaneous Immunoglobulin E Mice 03 medical and health sciences Subcutaneous injection Th2 Cells 0302 clinical medicine medicine Animals Immunology and Allergy Amino Acid Sequence Plant Proteins 030304 developmental biology Mice Inbred BALB C 0303 health sciences biology medicine.diagnostic_test business.industry Lasers Original Articles Immunotherapy transcutaneous allergy medicine.disease Asthma laser 3. Good health Cellular infiltration Bronchoalveolar lavage micropores 030228 respiratory system Immunoglobulin G biology.protein Female Cytokine secretion Nasal administration immunotherapy business |
| Zdroj: | Allergy |
| ISSN: | 1398-9995 0105-4538 |
| Popis: | Background Specific immunotherapy via the subcutaneous or oral route is associated with local and, in some cases, systemic side effects and suffers from low patient compliance. Due to its unique immunological features, the skin represents a promising target tissue for effective and painless treatment of type I allergy. The current study was performed to compare the efficacy of transcutaneous immunotherapy via laser-generated micropores to subcutaneous injection. Methods BALB/c mice were sensitized by intraperitoneal injection of recombinant grass pollen allergen Phl p 5 together with alum. Subsequently, lung inflammation was induced by repeated intranasal challenge. During the treatment phase, adjuvant-free Phl p 5 was applied in solution to microporated skin or was subcutaneously injected. Lung function and cellular infiltration; Phl p 5–specific serum levels of IgG1, IgG2a, and IgE; and cytokine levels in bronchoalveolar lavage fluids as well as in supernatants of splenocyte cultures were assessed. Results Both therapeutic approaches reduced airway hyperresponsiveness and leukocyte infiltration into the lungs. Whereas subcutaneous immunotherapy induced a systemic increase in Th2-associated cytokine secretion, transcutaneous application revealed a general downregulation of Th1/Th2/Th17 responses. Successful therapy was associated with induction of IgG2a and an increase in FOXP3+ CD4+ T cells. Conclusions Transcutaneous immunotherapy via laser microporation is equally efficient compared with conventional subcutaneous treatment but avoids therapy-associated boosting of systemic Th2 immunity. Immunotherapy via laser-microporated skin combines a painless application route with the high efficacy known from subcutaneous injections and therefore represents a promising alternative to established forms of immunotherapy. |
| Databáze: | OpenAIRE |
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