A transgenic mouse model for high content, cell cycle phenotype screening in live primary cells
| Autor: | Angela T. Hahn, Tobias Meyer, Richard O. Burney, Joshua T. Jones, Denise E. Leong, Alan I Lee, Mylene W.M. Yao |
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| Rok vydání: | 2007 |
| Předmět: |
Genetically modified mouse
Male Transgene Cell Mice Transgenic Biology Mice Species Specificity Pregnancy medicine Animals Humans Genetic Testing Molecular Biology Mitosis Cell Cycle Cell Biology Transfection Cell cycle Fibroblasts Cell biology Mice Inbred C57BL medicine.anatomical_structure Phenotype Mice Inbred DBA High-content screening Models Animal Female Chemical genetics Developmental Biology |
| Zdroj: | Cell cycle (Georgetown, Tex.). 6(18) |
| ISSN: | 1551-4005 |
| Popis: | High content cell-based genetic and small molecule library screens are powerful strategies in drug discovery and investigations of disease mechanisms. We report that primary cells derived from a transgenic mouse model expressing a fluorescence mitosis biosensor provide unambiguous phenotype readouts without the need for transfection or immunocytochemistry. Phenotype profiles of cell cycle disruption and of apoptosis are easily detectable at a single time point selected from time-lapse live fluorescence microscopy. Most importantly, this transgenic mouse model may be crossed with cancer mouse models to derive biosensor-expressing primary cancer cells for use in high content screening strategies targeting discovery of tumor-specific chemotherapeutic compounds. |
| Databáze: | OpenAIRE |
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