Status of Artemisinin Resistance in Malaria Parasite Plasmodium falciparum from Molecular Analyses of the Kelch13 Gene in Southwestern Nigeria
| Autor: | Daouda Ndiaye, Aida Sadikh Badiane, Praveen K. Bharti, Upasana Shyamsunder Singh, Nazia Anwar Ali, Aparup Das, Mary Aigbiremo Oboh, Hiasindh Ashmi Antony |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Article Subject 030231 tropical medicine lcsh:Medicine Southeast asian medicine.disease_cause General Biochemistry Genetics and Molecular Biology DNA sequencing 03 medical and health sciences 0302 clinical medicine Chloroquine parasitic diseases medicine Artemisinin Gene Genetics Mutation General Immunology and Microbiology biology lcsh:R Plasmodium falciparum General Medicine biology.organism_classification medicine.disease 030104 developmental biology Malaria medicine.drug |
| Zdroj: | BioMed Research International, Vol 2018 (2018) Oboh, M A, Ndiaye, D, Antony, H A, Badiane, A S, Singh, U S, Ali, N A, Bharti, P K & Das, A 2018, ' Status of Artemisinin Resistance in Malaria Parasite Plasmodium falciparum from Molecular Analyses of the Kelch13 Gene in Southwestern Nigeria ', BioMed Research International, vol. 2018 . https://doi.org/10.1155/2018/2305062 |
| ISSN: | 2314-6133 |
| DOI: | 10.1155/2018/2305062 |
| Popis: | Evolution and spread of malaria parasite Plasmodium falciparum capable of evading antimalarials are the prime concern to malaria control. The currently effective drug, artemisinin (ART), is under threat due to detection of ART-resistant P. falciparum parasites in the Southeast Asian countries. It has been shown that amino acid (AA) mutations at the P. falciparum Kelch13 (Pfk13) gene provide resistance to ART. Nigeria, a part of the Sub-Saharan Africa, is highly endemic to malaria, contributing quite significantly to malaria, and resistance to chloroquine (CQ) and sulfadoxine-pyrimethamine (SP) combination drugs has already been reported. Since artemisinin combined therapy (ACT) is the first-line drug for treatment of uncomplicated malaria in Nigeria and five amino acid mutations have been validated in the Pfk13 gene alongside with candidate mutations for ART resistance, we performed molecular surveillance for mutations (following PCR and DNA sequence analyses) in this gene from two southwestern states of Nigeria. Statistical analyses of DNA sequences were also performed following different evolutionary models. None of the different validated and candidate AA mutations of Pfk13 gene conferring resistance to ART could be detected in P. falciparum sampled in the two southwestern states of Nigeria. In addition, DNA sequencing and sequence analyses indicated neither evolutionary selection pressure on the Pfk13 gene nor association of mutations in Pfk13 gene with mutations of other three genes conferring resistance to CQ and SP. Therefore, based on the monomorphism at the Pfk13 gene and nonassociation of mutations of this gene with mutations in three other drug-resistant genes in malaria parasite P. falciparum, it can be proposed that malaria public health is not under immediate threat in southwestern Nigeria concerning ART resistance. |
| Databáze: | OpenAIRE |
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