Evaluating changes of blood flow in retina, choroid, and outer choroid in rats in response to elevated intraocular pressure by 1300 nm swept-source OCT

Autor: John C. Morrison, Ruikang K. Wang, William O. Cepurna, Jingjiang Xu, Yuandong Li, Shaozhen Song
Rok vydání: 2019
Předmět:
0301 basic medicine
medicine.medical_specialty
Intraocular pressure
Time Factors
genetic structures
Perfusion Imaging
Ocular hypertension
Glaucoma
Diagnostic Techniques
Ophthalmological
030204 cardiovascular system & hematology
Biochemistry
Article
03 medical and health sciences
0302 clinical medicine
Optical coherence tomography
Predictive Value of Tests
Rats
Inbred BN
Ophthalmology
medicine.artery
medicine
Animals
Intraocular Pressure
Retina
medicine.diagnostic_test
Choroid
business.industry
Microcirculation
Retinal Vessels
Equipment Design
Cell Biology
medicine.disease
eye diseases
Ciliary arteries
Disease Models
Animal
030104 developmental biology
medicine.anatomical_structure
Regional Blood Flow
Optic nerve
Ocular Hypertension
sense organs
Cardiology and Cardiovascular Medicine
business
Blood Flow Velocity
Tomography
Optical Coherence
Zdroj: Microvascular Research. 121:37-45
ISSN: 0026-2862
DOI: 10.1016/j.mvr.2018.09.003
Popis: We report the development of a 1300 nm swept-source optical coherence tomography (SS-OCT) system specifically designed to perform OCT imaging and optical microangiography (OMAG) in rat eyes in vivo and its use in evaluating the effects of intraocular pressure (IOP) elevation on ocular circulation. The swept laser is operated in single longitude mode with a 90 nm bandwidth centered at 1300 nm and 200 kHz A-line rate, providing remarkable sensitivity fall-off performance along the imaging depth, a larger field of view of 2.5 × 2.5 mm2 (approximately 35°), and more time-efficient imaging acquisition. The advantage of the SS-OCT/OMAG is highlighted by an increased imaging depth of the entire posterior thickness of optic nerve head (ONH) and its surrounding vascular anatomy, to include, for the first time in vivo, the vasculature at the scleral opening, allowing visualization of the circle of Zinn-Haller and posterior ciliary arteries (PCAs). Furthermore, the capillary-level resolution angiograms achieved at the retinal and choroidal layers over a larger field of view enable a significantly improved quantification of the response of vascular area density (VAD) to elevated IOP. The results indicate that reduction in perfusion of the choroid in response to elevated IOP is delayed compared to that seen in the retina; while choroidal VAD doesn't reach 50% of baseline until ~70 mmHg, the same effect is seen for the retinal VAD at ~60 mmHg. The superior image quality offered by SS-OCT may allow more comprehensive investigation of IOP-related ocular perfusion changes and their pathological roles in glaucomatous optic nerve damage.
Databáze: OpenAIRE
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