P2X7R and PANX-1 channel relevance in a zebrafish larvae copper-induced inflammation model
| Autor: | Fabiano Peres Menezes, Luiza Wilges Kist, F.O. de Marchi, Fernanda Bueno Morrone, Fernanda Fernandes Cruz, Maurício Reis Bogo |
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| Rok vydání: | 2019 |
| Předmět: |
Male
0301 basic medicine Purinergic P2X Receptor Antagonists Physiology Health Toxicology and Mutagenesis Interleukin-1beta chemistry.chemical_element Inflammation Pharmacology Toxicology medicine.disease_cause Biochemistry Connexins Connexon 03 medical and health sciences 0302 clinical medicine Acetamides Gene expression medicine Animals Zebrafish biology Probenecid Chemistry Interleukin Cell Biology General Medicine Zebrafish Proteins biology.organism_classification Copper Interleukin-10 Disease Models Animal Oxidative Stress 030104 developmental biology Gene Expression Regulation Larva Quinolines Female Receptors Purinergic P2X7 medicine.symptom Locomotion 030217 neurology & neurosurgery Oxidative stress medicine.drug |
| Zdroj: | Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology. 223:62-70 |
| ISSN: | 1532-0456 |
| Popis: | Copper is a metal that participates in several essential reactions in living organisms, and it has been used as an inflammatory inducing agent in zebrafish larvae. In this study, we evaluated the effect P2X7 receptor and/or pannexin channel 1 (PANX-1) blockage in this inflammation model. To perform the experiments, 7 dpf larvae were exposed to 10 μM of copper and treated with 100 μM probenecid, PANX-1 inhibitor, and/or 300 nM A740003, a P2X7R selective antagonist. Larvae survival was assessed up to 24 h after treatments. The evaluation of larvae behavior was evaluated after acute (4 h) and chronic (24 h) exposure. The parameters of locomotor activity measured were: mobile time, average speed, distance and turn angle. We analyzed the gene expression of the P2X7 receptor, PANX1a and PANX1b channels and interleukins IL-10 and IL-1b after 24 h of treatment. Treatments did not decrease larval survival in the time interval studied. Changes in larvae locomotion were observed after the longest time of exposure to copper and the treatment with probenecid was able to reverse part of the effects caused by copper. No significant difference was observed in the oxidative stress assays and probenecid and copper treatment decrease partially PANX1a gene expression groups. The data presented herein shows the relevance of the blockage of P2X7-PANX-1 in copper-induced inflammation. |
| Databáze: | OpenAIRE |
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