Exosomal‑miR‑10a derived from colorectal cancer cells suppresses migration of human lung fibroblasts, and expression of IL‑6, IL‑8 and IL‑1β
| Autor: | Ying Li, Xuan Zheng, Jun Zhang, Jian Wang, Jian-Hui Gan, Yuan-Ting Liu, Wanning Hu, Yan Liu, Ya-Qi Wang, Zhao-Yuan Wan, Yankun Liu, Yufeng Li |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Cancer Research Colorectal cancer Cell Interleukin-1beta colorectal cancer Exosomes Biochemistry Exosome 03 medical and health sciences 0302 clinical medicine Cell Movement Cell Line Tumor microRNA Genetics microRNA-10a Medicine Humans exosome Interleukin 8 RNA Neoplasm Molecular Biology Lung Oncogene business.industry Interleukin-6 Interleukin-8 lung fibroblasts Cancer Articles Fibroblasts medicine.disease Molecular medicine microenvironment digestive system diseases Coculture Techniques Neoplasm Proteins Gene Expression Regulation Neoplastic MicroRNAs 030104 developmental biology medicine.anatomical_structure Oncology 030220 oncology & carcinogenesis Cancer research Molecular Medicine business Colorectal Neoplasms |
| Zdroj: | Molecular Medicine Reports |
| ISSN: | 1791-3004 |
| Popis: | MicroRNAs (miRs) carried in exosomes serve an important role in the pre‑metastatic microenvironment and in intercellular interactions. However, the function of exosomal‑miR‑10a derived from primary colorectal cancer (CRC) cells on fibroblasts in the lung metastatic microenvironment of patients with CRC remains unclear. Reverse transcription‑quantitative PCR was performed using samples from patients with CRC, and demonstrated that the expression levels of miR‑10a were significantly lower in serum and cancer tissue samples from patients with CRC compared with in serum from healthy individuals and paired non‑cancerous tissues, respectively. In addition, the expression levels of miR‑10a were inversely associated with the invasion depth of CRC. Exosomal‑miR‑10a derived from CRC cells reduced the proliferative and migratory activities of primary normal human lung fibroblasts (NHLFs), and the expression levels of IL‑6, IL‑8 and IL‑1β in NHLFs. The present study provided insight into the phenotypic alterations of NHLFs induced by exosomal‑miR‑10a derived from CRC cells, which may aid understanding of the mechanism underlying the process of CRC lung metastasis. |
| Databáze: | OpenAIRE |
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