IrSPI, a Tick Serine Protease Inhibitor Involved in Tick Feeding and Bartonella henselae Infection
| Autor: | Muriel Vayssier-Taussat, José de la Fuente, Ruth C. Galindo, Xiang Ye Liu, Martine Cote, Sara Moutailler, Sarah Bonnet |
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| Přispěvatelé: | Biologie Moléculaire et Immunologie Parasitaires et Fongiques, École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Oklahoma State University [Stillwater], Universidad de Castilla-La Mancha (UCLM), China Scholarship Council (CSC), INRA, EU [FP7-261504 EDENext], Spanish Secretaria de Estado de Investigacion, Desarrollo e Innovacion, Ministerio de Economia y Competitividad [BFU2011-23896], École nationale vétérinaire - Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Oklahoma State University [Stillwater] (OSU), Universidad de Castilla-La Mancha = University of Castilla-La Mancha (UCLM), Ministerio de Economía y Competitividad (España), European Commission, China Scholarship Council |
| Rok vydání: | 2014 |
| Předmět: |
Bartonella
tique lcsh:Arctic medicine. Tropical medicine Serine Proteinase Inhibitors Ixodes ricinus lcsh:RC955-962 Médecine humaine et pathologie ComputingMilieux_LEGALASPECTSOFCOMPUTING Tick Salivary Glands Ticks Lyme disease Bartonella Infections parasitic diseases sang medicine Animals analyse du transcriptome Bartonella henselae biology lcsh:Public aspects of medicine Microbiology and Parasitology glande salivaire Ricinus Public Health Environmental and Occupational Health Biology and Life Sciences lcsh:RA1-1270 Babesiosis Feeding Behavior ixodes ricinus bacterial infections and mycoses biology.organism_classification medicine.disease Virology Microbiologie et Parasitologie 3. Good health [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology Infectious Diseases Host-Pathogen Interactions Human health and pathology Anaplasmosis bactérie pathogène Transcriptome [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology Bartonella Infection Research Article |
| Zdroj: | PLoS Neglected Tropical Diseases PLoS Neglected Tropical Diseases, Public Library of Science, 2014, 8 (7), ⟨10.1371/journal.pntd.0002993⟩ PLoS Neglected Tropical Diseases, Vol 8, Iss 7, p e2993 (2014) Plos Neglected Tropical Diseases 7 (8), . (2014) Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 1935-2735 1935-2727 |
| DOI: | 10.1371/journal.pntd.0002993 |
| Popis: | This is an open-access article distributed under the terms of the Creative Commons Attribution License.-- et al. Ixodes ricinus is the most widespread and abundant tick in Europe, frequently bites humans, and is the vector of several pathogens including those responsible for Lyme disease, Tick-Borne Encephalitis, anaplasmosis, babesiosis and bartonellosis. These tick-borne pathogens are transmitted to vertebrate hosts via tick saliva during blood feeding, and tick salivary gland (SG) factors are likely implicated in transmission. In order to identify such tick factors, we characterized the transcriptome of female I. ricinus SGs using next generation sequencing techniques, and compared transcriptomes between Bartonella henselae-infected and non-infected ticks. High-throughput sequencing of I. ricinus SG transcriptomes led to the generation of 24,539 isotigs. Among them, 829 and 517 transcripts were either significantly up- or down-regulated respectively, in response to bacterial infection. Searches based on sequence identity showed that among the differentially expressed transcripts, 161 transcripts corresponded to nine groups of previously annotated tick SG gene families, while the others corresponded to genes of unknown function. Expression patterns of five selected genes belonging to the BPTI/Kunitz family of serine protease inhibitors, the tick salivary peptide group 1 protein, the salp15 super-family, and the arthropod defensin family, were validated by qRT-PCR. IrSPI, a member of the BPTI/Kunitz family of serine protease inhibitors, showed the highest up-regulation in SGs in response to Bartonella infection. IrSPI silencing impaired tick feeding, as well as resulted in reduced bacterial load in tick SGs. This study provides a comprehensive analysis of I. ricinus SG transcriptome and contributes significant genomic information about this important disease vector. This in-depth knowledge will enable a better understanding of the molecular interactions between ticks and tick-borne pathogens, and identifies IrSPI, a candidate to study now in detail to estimate its potentialities as vaccine against the ticks and the pathogens they transmit. XYL was supported by funds from the China Scholarship Council (CSC). This work was funded by INRA and EU grant FP7-261504 EDENext and is catalogued by the EDENext Steering Committee as EDENext037 (http://www.edenext.eu). This work was also partially supported by the Spanish Secretaría de Estado de Investigación, Desarrollo e Innovación, Ministerio de Economía y Competitividad project BFU2011-23896. |
| Databáze: | OpenAIRE |
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