Sensitive methods for the determination of ester sulfate in biological systems
Autor: | Nicola Di Ferrante, Leonard C. Ginsberg |
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Rok vydání: | 1977 |
Předmět: |
Placenta
Inorganic chemistry Oligosaccharides Sulfuric Acid Esters Kidney Biochemistry chemistry.chemical_compound Pregnancy Spectrophotometry Methods medicine Animals Humans Cells Cultured Skin Chromatography medicine.diagnostic_test Heparin Chemistry Microchemistry Chondroitin Sulfates Dextrans Sulfuric acid Fibroblasts Sulfuric Acids Benzidine Inorganic sulfate Ester sulfate Female Indicators and Reagents medicine.drug |
Zdroj: | Biochemical Medicine. 17:80-86 |
ISSN: | 0006-2944 |
DOI: | 10.1016/0006-2944(77)90011-4 |
Popis: | The benzidine and rhodizonate methods for the assay of inorganic sulfate have been modified in order to increase their sensitivity, rapidity, and safety. With these modifications, amounts of inorganic sulfate ranging between 0.15 and 15 μg (1.56 to 156 nmoles) may be conveniently measured. At this level of sensitivity, the methods may be used for the assay of several sulfohydrolases of biological relevance without using 35SO4-labeled substrates. |
Databáze: | OpenAIRE |
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