Sensitive methods for the determination of ester sulfate in biological systems

Autor: Nicola Di Ferrante, Leonard C. Ginsberg
Rok vydání: 1977
Předmět:
Zdroj: Biochemical Medicine. 17:80-86
ISSN: 0006-2944
DOI: 10.1016/0006-2944(77)90011-4
Popis: The benzidine and rhodizonate methods for the assay of inorganic sulfate have been modified in order to increase their sensitivity, rapidity, and safety. With these modifications, amounts of inorganic sulfate ranging between 0.15 and 15 μg (1.56 to 156 nmoles) may be conveniently measured. At this level of sensitivity, the methods may be used for the assay of several sulfohydrolases of biological relevance without using 35SO4-labeled substrates.
Databáze: OpenAIRE