p185 overexpression in 220 samples of breast cancer undergoing primary surgery: comparison with c-erbB-2 gene amplification
| Autor: | A. Lortholary, G Lorimier, A Daver, I Dalifard, S Gosse-Brun, J Goussard, F Larra |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Adult
Oncology medicine.medical_specialty animal structures Receptor ErbB-2 Statistics as Topic Population Breast Neoplasms Adenocarcinoma Biology medicine.disease_cause Immunoenzyme Techniques Breast cancer Internal medicine Genetics medicine Humans Receptor education Aged Neoplasm Staging Retrospective Studies Southern blot Aged 80 and over education.field_of_study Oncogene Gene Amplification Cancer General Medicine Genes erbB-2 Middle Aged Cell cycle medicine.disease Gene Expression Regulation Neoplastic Receptors Estrogen Female Lymph Nodes Receptors Progesterone Carcinogenesis |
| Zdroj: | International Journal of Molecular Medicine. |
| ISSN: | 1791-244X 1107-3756 |
| DOI: | 10.3892/ijmm.1.5.855 |
| Popis: | In breast cancer, DNA amplification of the oncogene c-erbB-2, encoding for the p185 protein, is associated with a poor prognosis. A retrospective study on a population of 220 cases of primary breast cancer permitted a quantitative measure of p185 oncoprotein overexpression by an immunoenzymetric assay and the determination of c-erbB-2 amplification by the Southern blot method. A correlation existed between the two measurements (r=0.85) using the double cut-off: DNA 2 copies and p185 400 U/mg protein, and only 2.7% of the cases were discordant. 13.2% of the tumors showed p185 overexpression. The percentage of tumors overexpressing p185 was significantly different between the groups with amplified and non-amplified c-erbB-2. We observed a significant correlation between p185 levels and tumor grade (p=0.03), and an inverse correlation with hormonal receptors (p=0.0001). The p185 assay could be an additional prognostic factor to better define patient subgroups with node negative, grade II, and positive or negative hormonal receptors. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|