Isolation, identification of sludge-lysing strain and its utilization in thermophilic aerobic digestion for waste activated sludge
| Autor: | Toshinari Maeda, Hiroaki I. Ogawa, Shoichiro Matsumoto, Qunhui Wang, Hongzhi Ma, Xuesong Li |
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| Rok vydání: | 2009 |
| Předmět: |
Environmental Engineering
Microorganism Bioengineering Biology Water Purification Microbiology Bacterial Proteins Water Pollutants Aerobic digestion Food science Waste Management and Disposal Phylogeny Sewage Renewable Energy Sustainability and the Environment Thermophile Temperature Sterilization General Medicine Hydrogen-Ion Concentration Biodegradation Bacteria Aerobic Biodegradation Environmental Activated sludge Sewage treatment Digestion Sludge Peptide Hydrolases |
| Zdroj: | Bioresource Technology. 100:2475-2481 |
| ISSN: | 0960-8524 |
| DOI: | 10.1016/j.biortech.2008.12.019 |
| Popis: | A strain of sludge-lysing bacteria was isolated from waste activated sludge (WAS) in this study. The result of 16S rRNA gene analysis demonstrated that it was a species of new genus Brevibacillus (named Brevibacillus sp. KH3). The strain could release the protease with molecule weight of about 40 kDa which could enhance the efficiency of sludge thermophilic aerobic digestion. During the sterilized sludge digestion experiment inoculated with Brevibacillus sp. KH3, the maximum protease activity was 0.41 U/ml at pH 8 and 50 degrees C, and maximum TSS removal ratio achieved 32.8% after 120 h digestion at pH 8 and 50 degrees C. In the case of un-sterilized sludge digestion inoculated with Brevibacillus sp. KH3, TSS removal ratio in inoculated-group was 54.8%, increasing at 11.86% compared with un-inoculation (46.2%). The result demonstrated that inoculation of Brevibacillus sp. KH3 could help to degrade the EPS and promote the collapse of cells and inhibit the growth of certain kinds of microorganisms. It indicated that Brevibacillus sp. KH3 strain had a high potential to enhance WAS-degradation efficiency in thermophilic aerobic digestion. |
| Databáze: | OpenAIRE |
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