Differential effects of intravenous anesthetics on hepatosplanchnic microcirculation in rats: Sidestream dark-field imaging study
| Autor: | R Parizkova, Jindrich Samek, Zdenek Turek, Milan Kaška, Christian Lehmann, Vladimir Cerny |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Male
Pentobarbital Physiology Midazolam medicine.medical_treatment Fentanyl Microcirculation Bolus (medicine) Ileum Physiology (medical) medicine Animals Ketamine Rats Wistar Thiopental Propofol Saline business.industry Hematology Rats Liver Anesthesia Blood Gas Analysis Cardiology and Cardiovascular Medicine business Anesthetics Intravenous medicine.drug |
| Zdroj: | Clinical Hemorheology and Microcirculation. 51:213-223 |
| ISSN: | 1386-0291 |
| Popis: | This study aimed to investigate the effects of intravenous anesthetics on hepatosplanchnic microcirculation in laparotomized mechanically ventilated rats using Sidestream Dark-field (SDF) imaging. Thirty male Wistar rats were divided into 5 groups (n = 6 each). All rats were initially anesthetized with 60 mg/kg pentobarbital (i.p.) for instrumentation. This was followed by either ketamine, propofol, thiopental, midazolam or saline+fentanyl (iv bolus over 5 min and then maintenance over 90 min). SDF imaging of the liver and distal ileum microcirculation was performed at the baseline and at t = 5, 35, 65 and 95 min. In propofol group there was increase of functional sinusoidal density (FSD) following induction (+25%, P < 0.05) and maintenance at t = 95 min (+10.3%, P < 0.05), in ketamine and midazolam group decrease of FSD was observed after induction (-20.4%, P < 0.05; -10.1%, P < 0.05) and during maintenance at t = 65 min (-11.6%, P < 0.05; -11.4%, P < 0.05) when compared to baseline. Following induction with propofol functional capillary density (FCD) of ileal longitudinal muscle layer increased (+10.6%, P < 0.05) and returned to baseline values during maintenance. Ketamine and midazolam decreased FCD of longitudinal layer after induction (-24.6%, P < 0.05; -21.1%, P < 0.05) and remained decreased during maintenance at t = 95 min (-10.8%, P < 0.05; -15.5%, P < 0.05). In thiopental and control group, changes in microcirculatory parameters were not significant throughout the study. In conclusion, intravenous anesthetics affect the hepatosplanchnic microcirculation differentially, propofol has shown protective effect on the liver and intestinal microcirculation. |
| Databáze: | OpenAIRE |
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