The Effect of 3′-Hydroxy-3,4,5,4′-Tetramethoxy -stilbene, the Metabolite of the Resveratrol Analogue DMU-212, on the Motility and Proliferation of Ovarian Cancer Cells
| Autor: | Marcin Rucinski, Hanna Piotrowska-Kempisty, Małgorzata Józkowiak, Piotr Ramlau, Paulina Skupin-Mrugalska, Andrzej Nowicki, Violetta Krajka-Kuzniak, Marcin Wierzchowski, Jadwiga Jodynis-Liebert |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
proliferation
Motility Resveratrol migration Catalysis Article Metastasis Inorganic Chemistry Transcriptome lcsh:Chemistry chemistry.chemical_compound Kruppel-Like Factor 4 Cell Movement Cell Line Tumor Stilbenes medicine Cytotoxic T cell Humans Physical and Theoretical Chemistry Molecular Biology lcsh:QH301-705.5 Spectroscopy Cell Proliferation Ovarian Neoplasms Cell growth Organic Chemistry General Medicine medicine.disease dmu-214 Computer Science Applications resveratrol analogue ovarian cancer chemistry lcsh:Biology (General) lcsh:QD1-999 KLF4 Cancer research Female Ovarian cancer |
| Zdroj: | International Journal of Molecular Sciences, Vol 21, Iss 3, p 1100 (2020) International Journal of Molecular Sciences Volume 21 Issue 3 |
| ISSN: | 1422-0067 |
| Popis: | Targeting tumor cell motility and proliferation is an extremely important challenge in the prevention of metastasis and improving the effectiveness of cancer treatment. We recently published data revealing that DMU-214, the metabolite of firmly cytotoxic resveratrol analogue DMU-212, exerted significantly higher biological activity than the parent compound in ovarian cancer cells. The aim of the present study was to assess the molecular mechanism of the potential anti-migration and anti-proliferative effect of DMU-214 in ovarian cancer cell line SKOV-3. We showed that DMU-214 reduced the migratory capacity of SKOV-3 cells. The microarray analysis indicated ontology groups of genes involved in processes of negative regulation of cell motility and proliferation. Furthermore, we found DMU-214 triggered changes in expression of several migration- and proliferation-related genes (SMAD7, THBS1, IGFBP3, KLF4, Il6, ILA, SOX4, IL15, SRF, RGCC, GPR56) and proteins (GPR56, RGCC, SRF, SMAD7, THBS1), which have been shown to interact to each other to reduce cell proliferation and motility. Our study showed for the first time that DMU-214 displayed anti-migratory and anti-proliferative activity in SKOV-3 ovarian cancer cells. On the basis of whole transcriptome analysis of these cells, we provide new insight into the role of DMU-214 in inhibition of processes related to metastasis. |
| Databáze: | OpenAIRE |
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