Long-term SARS-CoV-2 infection associated with viral dissemination in different body fluids Including bile in two patients with acute cholecystitis
| Autor: | Massimo Andreoni, Ada Bertoli, Elisabetta Teti, Francesca Ceccherini-Silberstein, Rossana Scutari, Vincenzo Malagnino, Leandro Siragusa, Michele Grande, M. Manuelli, Daniele Sforza, Loredana Sarmati, Claudia Alteri, L. Piermatteo, Maria Concetta Bellocchi, Valentina Svicher, Marco Iannetta, Romina Salpini, Patrizia Saccomandi |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RT-PCR Case Report Urine General Biochemistry Genetics and Molecular Biology 03 medical and health sciences 0302 clinical medicine Acute cholecystitis Medicine Digital polymerase chain reaction Viral rna 030212 general & internal medicine lcsh:Science Ecology Evolution Behavior and Systematics business.industry SARS-CoV-2 fungi Paleontology RNA Droplet Digital PCR Settore MED/17 Settore MED/18 030104 developmental biology Real-time polymerase chain reaction Space and Planetary Science Rna quantification Immunology lcsh:Q RNA quantification business body compartment |
| Zdroj: | Life Life, Vol 10, Iss 302, p 302 (2020) |
| Popis: | Our study aimed to investigate the kinetics of SARS-CoV-2 RNA in bile and in different body fluids of two SARS-CoV-2 positive patients with acute cholecystitis by innovative droplet digital PCR (ddPCR) assays. For each patient, nasopharyngeal- and rectal swabs, bile, urine, and plasma samples were collected at different time points for SARS-CoV-2 RNA quantification by two ddPCR assays. For both patients, ddPCR revealed persistent and prolonged detection of viral RNA in the nasopharyngeal swab despite triple-negative or single-positive results by qRT-PCR. In Patient 1, SARS-CoV-2 RNA dropped more rapidly in bile and rectal-swab and declined slowly in nasopharyngeal swab and plasma, becoming undetectable in all compartments 97 days after symptoms started. Conversely, in patient 2, SARS-CoV-2 RNA was detected, even if at low copies, in all body samples (with the exception of urine) up to 75 days after the onset of symptoms. This study highlights that SARS-CoV-2 RNA can persist for a prolonged time in respiratory samples and in several biological samples despite negativity to qRT-PCR, supporting SARS-CoV-2’s ability to provoke persistent and disseminated infection and therefore to contribute to extra-pulmonary clinical manifestations. |
| Databáze: | OpenAIRE |
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