The effect of the transfusion of stored RBCs on intestinal microvascular oxygenation in the rat
| Autor: | Martin Siegemund, Christiaan P. Henny, Adrianus Trouwborst, Jasper van Bommel, Dirk de Korte, Arthur J. Verhoeven, Albert Lind, Can Ince |
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| Přispěvatelé: | Other departments |
| Jazyk: | angličtina |
| Rok vydání: | 2001 |
| Předmět: |
Male
Pathology medicine.medical_specialty Resuscitation Erythrocytes Cell Respiration Immunology Hemodynamics Shock Hemorrhagic Microcirculation Andrology Oxygen Consumption Erythrocyte Deformability hemic and lymphatic diseases medicine Animals Immunology and Allergy Rats Wistar O2 consumption business.industry hemic and immune systems Hematology Oxygenation Rats Intestines Disease Models Animal Red blood cell medicine.anatomical_structure Tissue oxygenation Blood Preservation Hemorrhagic shock Blood Gas Analysis Erythrocyte Transfusion business circulatory and respiratory physiology |
| Zdroj: | Transfusion, 41(12), 1515-1523. Wiley-Blackwell |
| ISSN: | 0041-1132 |
| DOI: | 10.1046/j.1537-2995.2001.41121515.x |
| Popis: | BACKGROUND: Although it is known that the transfusion of stored RBCs does not always improve tissue O2 consumption under conditions of limited tissue oxygenation, the efficiency of O2 delivery to the microcirculation by stored RBCs has never been determined. STUDY DESIGN AND METHODS: In a rat hemorrhagic shock model, the effects of resuscitation with fresh or 28-day-old RBCs stored in CPD plasma, saline-adenine-glucose-mannitol, and CPDA-1 plasma were investigated. Systemic hemodynamic and intestinal oxygenation measures were monitored. Intestinal microvascular PO2 was determined with the O2-dependent quenching of palladium-porphyrin phosphorescence, and the RBC deformability was measured with a Laser-assisted optic rotational cell analyzer. RESULTS: Hemodynamic and oxygenation measures were significantly decreased during hemorrhagic shock. Intestinal oxygen consumption and mesenteric venous pO2 were restored with the transfusion of both fresh and stored RBCs, except for CPD-stored RBCs. The intestinal microvascular pO2 improved only with the transfusion of fresh RBCs. Deformability of the stored RBCs was significantly decreased. CONCLUSION: In contrast to that of fresh RBCs, the transfusion of stored RBCs did not restore the microcirculatory oxygenation, possibly because of impaired O2 unloading, but, except for CPD-stored RBCs, the storage-induced changes were not enough to impair intestinal VO2 and mesenteric venous pO2. |
| Databáze: | OpenAIRE |
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