Calcium channel α2δ1 subunit mediates spinal hyperexcitability in pain modulation
| Autor: | Xiulin Zhang, Anthony H. Dickenson, Chun-Ying Li, Kang-Wu Li, Elizabeth A. Matthews, Jimmy Gross, Ambereen Kurwa, Z. David Luo, Michael S. Gold, Amin Boroujerdi, Guoping Feng |
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| Rok vydání: | 2006 |
| Předmět: |
Pain Threshold
Central nervous system Pain Mice Transgenic Stimulation Article Mice chemistry.chemical_compound Dorsal root ganglion Ganglia Spinal medicine Animals business.industry Calcium channel Chronic pain medicine.disease Sensory neuron Posterior Horn Cells Protein Subunits Anesthesiology and Pain Medicine medicine.anatomical_structure nervous system Neurology chemistry Hyperalgesia Neuropathic pain Calcium Channels Neurology (clinical) business Neuroscience Gabapentinoid |
| Zdroj: | Pain. 125:20-34 |
| ISSN: | 0304-3959 |
| DOI: | 10.1016/j.pain.2006.04.022 |
| Popis: | Mechanisms of chronic pain, including neuropathic pain, are poorly understood. Upregulation of voltage-gated calcium channel (VGCC) alpha2delta1 subunit (Ca(v)alpha2delta1) in sensory neurons and dorsal spinal cord by peripheral nerve injury has been suggested to contribute to neuropathic pain. To investigate the mechanisms without the influence of other injury factors, we have created transgenic mice that constitutively overexpress Ca(v)alpha2delta1 in neuronal tissues. Ca(v)alpha2delta1 overexpression resulted in enhanced currents, altered kinetics and voltage-dependence of VGCC activation in sensory neurons; exaggerated and prolonged dorsal horn neuronal responses to mechanical and thermal stimulations at the periphery; and pain behaviors. However, the transgenic mice showed normal dorsal horn neuronal responses to windup stimulation, and behavioral responses to tissue-injury/inflammatory stimuli. The pain behaviors in the transgenic mice had a pharmacological profile suggesting a selective contribution of elevated Ca(v)alpha2delta1 to the abnormal sensations, at least at the spinal cord level. In addition, gabapentin blocked VGCC currents concentration-dependently in transgenic, but not wild-type, sensory neurons. Thus, elevated neuronal Ca(v)alpha2delta1 contributes to specific pain states through a mechanism mediated at least partially by enhanced VGCC activity in sensory neurons and hyperexcitability in dorsal horn neurons in response to peripheral stimulation. Modulation of enhanced VGCC activity by gabapentin may underlie at least partially its antihyperalgesic actions. |
| Databáze: | OpenAIRE |
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