A nose-only apparatus for airborne delivery of Mycobacterium tuberculosis to mice: calibration of biological parameters
| Autor: | Damien Descroix, Gilles Vergnaud, Gilles Marchal, Iroudayanadin Delamanche, Céline Louveau, Laurence Garnier, P Chavarot, Françoise Ramisse |
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| Přispěvatelé: | Laboratoire de Référence des Mycobactéries, Institut Pasteur [Paris], Département d'analyse biologique [CEB], CEB Centre d'études du Bouchet, Département d'évaluation des systèmes de défense [CEB], Département d'analyse chimique [CEB], SPnum vsh, Direction générale de l'Armement (DGA), The work on biological risk assessment at CEB was supported by the French Ministry of Defence, Institut Pasteur [Paris] (IP) |
| Jazyk: | angličtina |
| Rok vydání: | 2005 |
| Předmět: |
Tuberculosis
[SDV]Life Sciences [q-bio] Immunology Microbiology Mycobacterium tuberculosis Mice 03 medical and health sciences medicine Animals BioHazard Particle Size Tuberculosis Pulmonary Pathogen 030304 developmental biology Aerosols Mice Inbred BALB C 0303 health sciences biology Inhalation 030306 microbiology Nebulizers and Vaporizers Mycobacterium smegmatis Containment of Biohazards biology.organism_classification medicine.disease 3. Good health Disease Models Animal Infectious Diseases medicine.anatomical_structure [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology Female [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology Respiratory tract Mycobacterium |
| Zdroj: | Microbes and Infection Microbes and Infection, Elsevier, 2005, 7 (3), pp.457-66. ⟨10.1016/j.micinf.2004.11.019⟩ Microbes and Infection, 2005, 7 (3), pp.457-66. ⟨10.1016/j.micinf.2004.11.019⟩ |
| ISSN: | 1286-4579 |
| DOI: | 10.1016/j.micinf.2004.11.019⟩ |
| Popis: | International audience; Mycobacterium tuberculosis is the main cause of tuberculosis and is still a public health concern worldwide. This mycobacterium is transmitted through aerosols from human beings suffering from pulmonary tuberculosis to susceptible persons. To study this natural route of infection, we designed a new nose-only aerosol apparatus--system of aerosolisation of microorganisms (SAM)--in a carefully designed biohazard facility. For safety reasons, Mycobacterium smegmatis was first used to calibrate several parameters, such as inoculum density, atmospheric conditions (i.e. hygrometry) and particle size distribution. We present evidence that our apparatus is totally adapted to airborne delivery; the particle size of generated aerosol ranges from 1 to 7 microm, which is ideal for an infection by inhalation. We found that 99% of generated particles ( |
| Databáze: | OpenAIRE |
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