New use of old medicine: Nifedipine acts on the TRP family and inflammatory proteins in the treatment of chilblain
| Autor: | Mei Xie, Xiuya Li, Yongqiang Zhou, Helv Yan, Chunli Zhao, Ting Li |
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| Rok vydání: | 2021 |
| Předmět: |
Drug
Vascular Endothelial Growth Factor A Erythema Nifedipine media_common.quotation_subject chemistry.chemical_element Pannus Pharmacology Calcium Critical Care and Intensive Care Medicine Rats Sprague-Dawley 030207 dermatology & venereal diseases 03 medical and health sciences Transient receptor potential channel 0302 clinical medicine Medicine Animals Humans media_common business.industry Interleukin-6 Tumor Necrosis Factor-alpha Antagonist 030208 emergency & critical care medicine General Medicine medicine.disease Rats Chilblains chemistry Emergency Medicine Immunohistochemistry Surgery medicine.symptom business Burns medicine.drug |
| Zdroj: | Burns : journal of the International Society for Burn Injuries. 48(2) |
| ISSN: | 1879-1409 |
| Popis: | As a calcium antagonist, the mechanism of nifedipine for treating chilblain has not been reported. In the present study, we established the chilblain model by using -20 ℃ 95% ethanol to freeze the right back foot of SD rats, and investigated the effects of this drug. Hematoxylin-eosin (HE) examination indicated most of pannus in the skin tissue of chilblain rats had disappeared, and the local inflammatory cells were also greatly reduced when given nifedipine at 15.0 mg/kg/d. The enzyme-linked immunosorbent assay (ELISA) revealed that nifedipine inhibited release of inflammatory factors TNF-α, IL-6, IL-1β and VEGF in serum. The RT-PCR analysis showed that nifedipine down regulated mRNA levels of TRPC-6 and VEGF in skin tissue. Furthermore, immunohistochemical examination showed nifedipine inhibited expression of IL-1β, IL-6, and TNF-α inflammatory protein and further inhibited expression of TRP (transient receptor potential) family proteins TRPM-7, TRPC-1, TRPC-3 and TRPC-6 and reduced expression of VEGF in skin and relieved erythema and oedema. This study demonstrated that nifedipine as an old medicine can be new use for the treatment of chilblain by acting on TRPs family and inflammatory proteins. |
| Databáze: | OpenAIRE |
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