Long non-coding RNAE330013P06 promotes progression of breast cancer with type 2 diabetes

Autor: Bo Zhang, Li Peng, Xiaoping Li, JinmingXue, Peng-Cheng Shi, Jun Yang, Run-Qi Chen, Fu-Guo Tian, Hai-Ming Wu, Liang Yao, Fei Luo, Wengfu Yang, Qianjun Wang, Yongmin Miao, Wangfu Wang, Yan Zhang
Rok vydání: 2019
Předmět:
0301 basic medicine
Microbiology (medical)
Oncology
medicine.medical_specialty
Clinical Biochemistry
Apoptosis
Breast Neoplasms
Type 2 diabetes
03 medical and health sciences
0302 clinical medicine
Breast cancer
breast cancer
Downregulation and upregulation
Cell Movement
Internal medicine
Diabetes mellitus
Cell Line
Tumor
medicine
long non‐coding RNAE330013P06
Immunology and Allergy
Humans
cell growth
Risk factor
Family history
skin and connective tissue diseases
Research Articles
Aged
Cell Proliferation
diabetes
business.industry
Biochemistry (medical)
Cell Cycle
Public Health
Environmental and Occupational Health
Cancer
Hematology
medicine.disease
Prognosis
Up-Regulation
Gene Expression Regulation
Neoplastic
Medical Laboratory Technology
030104 developmental biology
Diabetes Mellitus
Type 2
030220 oncology & carcinogenesis
Cancer cell
Female
RNA
Long Noncoding
business
Research Article
Zdroj: Journal of Clinical Laboratory Analysis
ISSN: 1098-2825
Popis: Background In previous research, we found diabetes rather than obesity was an independent risk factor of breast cancer. However, why diabetes could lead to increased risk of breast cancer patients remains elusive. Long non‐coding RNAE330013P06 has been shown to be upregulated in diabetes, and long non‐coding RNAs generally promote progression of cancer. Methods About 200 specimens of breast patients were obtained in previous clinical trial; 34 samples diagnosed as type 2 diabetes in breast cancer patient were enrolled in this research. Blood samples from 36 patients diagnosed as breast cancer without diabetes; 35 diabetic patients and 35 healthy peoples were obtained as control. All blood samples were measured by quantitative real‐time PCR (qRT‐PCR). Invasion and migration were tested by Transwell assay. Cell proliferation assay was tested by CCK‐8. Protein analysis was determined by Western blot. Results Compared with breast cancer patients without diabetes, diabetic patients without breast cancer and healthy peoples, LncRNAE330013P06 was upregulated in breast cancer patient with diabetes. Furthermore, of 34 breast patients, high LncRNAE330013P06 expression was significantly associated with family history, tumor‐node‐metastasis stage and lymph node metastasis. E33 promoted cancer cell growth in vitro via downregulation of P53. Conclusion Upregulation of LncRNAE330013P06 driven by type 2 diabetes is one of the factors which promoted progression of breast cancer.
Databáze: OpenAIRE
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