Processing and characterization of canine mixed mammary tumor using transmission electron microscopy
| Autor: | Beltrán Audrey, Debut Alexis, V Vaca Andrea, Ortiz Julio, Proaño-Pérez Freddy |
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| Rok vydání: | 2017 |
| Předmět: |
Microscopy
Electron Scanning Transmission 0301 basic medicine medicine.medical_specialty Pathology Histology 040301 veterinary sciences Biopsy 030106 microbiology Mammary gland Uranyl acetate Mammary Neoplasms Animal Biology 0403 veterinary science 03 medical and health sciences chemistry.chemical_compound Dogs Mammary Glands Animal Microscopy Electron Transmission medicine Animals Neoplasm Dog Diseases Instrumentation Mixed tumor medicine.diagnostic_test 04 agricultural and veterinary sciences medicine.disease Neoplasms Complex and Mixed Medical Laboratory Technology medicine.anatomical_structure chemistry Transmission electron microscopy Ultrastructure Female Histopathology Anatomy |
| Zdroj: | Microscopy Research and Technique. 80:1229-1233 |
| ISSN: | 1059-910X |
| DOI: | 10.1002/jemt.22921 |
| Popis: | Canine mammary gland tumors represent the second most frequent type of neoplasm in dogs, being an important problem within veterinary medical field. Canine mixed mammary tumors are the most common; the use of a transmission electron microscope (TEM) can contribute as a tool in its diagnosis by determining the characteristics of cellular components from numerous neoplasms. The aim of this study was to characterize cytologically canine mammary mixed tumor by the use of the TEM. A biopsy collected from an 11 years old bitch Shih-Tzu and analyzed by histopathology was used for ultrastructural analysis. Specimens obtained were double stained using uranyl acetate and lead citrate prior to observation in the TEM. The protocol established to transmission electron microscopy observation allowed the identification of main cellular characteristics of canine mixed mammary tumors; however, it was not possible a detailed visualization of the organelles due to the preservation of the biopsy in formaldehyde. |
| Databáze: | OpenAIRE |
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