FunctionalGUSassay ofGRAStranscription factor fromMedicago truncatula
| Autor: | Mariana Radkova, Lyuben Zagorchev, Anelia Iantcheva, Miglena Revalska |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0303 health sciences Plant growth lcsh:Biotechnology fungi food and beverages GUS reporter system medicago truncatula Biology gras transcription factor biology.organism_classification 01 natural sciences Salinity stress Medicago truncatula gus assay Cell biology 03 medical and health sciences lcsh:TP248.13-248.65 Gene family Signal transduction Transcription factor salinity stress 030304 developmental biology 010606 plant biology & botany Biotechnology |
| Zdroj: | Biotechnology & Biotechnological Equipment, Vol 33, Iss 1, Pp 1187-1194 (2019) |
| ISSN: | 1314-3530 1310-2818 |
| Popis: | GRAS gene family encodes transcription factors (TFs) which plays a vital role in different processes of plant growth and development of stems and roots, phytohrome-A signal transduction (PAT), gibberellic acid signal transduction (GA), plant response to biotic and abiotic stress conditions and disease resistance. They could be induced by salt and drought stress and in response to pathogen attack. In this research we investigated the expression pattern of the gene encoding MtGRAS TF (Medtr2g026250) by functional GUS assay in the model legume species Medicago truncatula. Transcriptional reporter lines carrying the promoter region of the gene encoding MtGRAS TF fused to GUS and GFP reporter genes were obtained via Agrobacterium-transformation of suspension cultures. The activity of the GUS reporter gene was detected in various parts and tissues of T1 generation of transformed plants, as well as under salinity stress. |
| Databáze: | OpenAIRE |
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