Molecular labels for analysis of amines and diols by spray based ionization-mass spectrometry
| Autor: | Stephen T. Ayrton, Michael Pugia, R. G. Cooks |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Aqueous solution
Electrospray ionization 010401 analytical chemistry Acetal 010402 general chemistry Mass spectrometry 01 natural sciences Biochemistry 0104 chemical sciences Analytical Chemistry chemistry.chemical_compound Hydrolysis chemistry Electrochemistry Anhydrous Environmental Chemistry Organic chemistry Pyridinium Ionization mass spectrometry Spectroscopy |
| Zdroj: | The Analyst. 141:5398-5403 |
| ISSN: | 1364-5528 0003-2654 |
| Popis: | A family of imidazolium and pyridinium salts was synthesized for the purpose of labeling amines and 1,2-diols for highly sensitivite analysis by mass spectrometry. The chosen mass labels are shown to serve as effective reporters when bound to particles functionalized with amines or 1,2-diols and the binding is reversible. The straightforward synthetic route allows analogous internal standards to be generated quickly. Solvents amenable to electrospray ionization facilitate the rapid liberation of imidazolium and pyridinium mass labels from particles by hydrolysis in aqueous acid, while the acetal bond remains stable in anhydrous or buffered aqueous solution. Detection of the labels is demonstrated at 1 nM and quantitation of mass labels released from particles is also demonstrated. |
| Databáze: | OpenAIRE |
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