Influence of polymer membrane porosity on C3A hepatoblastoma cell adhesive interaction and function
| Autor: | George Altankov, Thomas Weigel, Natalia Krasteva, Wolfgang Albrecht, Thomas Groth, Michael Schossig, Barbara Seifert |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Hepatoblastoma
Materials science Polymers Biophysics Bioengineering Cell morphology Biomaterials Focal adhesion Cytochrome P-450 Enzyme System Cell Line Tumor Cell Adhesion Humans Porosity Cell adhesion biology Cell growth Liver Neoplasms Membranes Artificial Adhesion Cell biology Fibronectin Membrane Mechanics of Materials Microscopy Electron Scanning Ceramics and Composites biology.protein |
| Zdroj: | Biomaterials. 25:2467-2476 |
| ISSN: | 0142-9612 |
| DOI: | 10.1016/j.biomaterials.2003.09.031 |
| Popis: | The effect of the porosity of acrylonitrile-N-vinylpyrrolidone copolymer membranes on human C3A hepatoblastoma cell adhesive interaction and functioning is investigated on four membranes with an average pore size ranging between 6 and 12 nm. Adhesion of C3A cells was quantified and characterized by studying overall cell morphology and focal adhesion formation. Cell-cell interactions were characterized by E-cadherin expression and organization. Cell growth, fibronectin synthesis and cytochrome P450 activity were estimated as criteria of functional cell activity. The results suggest that membrane porosity influences the initial cell-surface interactions since an increasing pore size augmented cell adhesion and aggregate formation. Cell growth after 7 d was diminished on membranes with an average pore size of 12 nm. The activity of P450 measured by 7-ethoxycoumarin conversion at day 7 was influenced by membrane topography representing a clear optimum in the range of 7-10 nm pore size. These results indicate that membrane porosity is a determinant for the function of hepatocytes in extracorporal liver assist devices. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect