Panel of serum miRNAs as potential non-invasive biomarkers for pancreatic ductal adenocarcinoma

Autor: Deepak Gunjan, Anoop Saraya, Shyam S. Chauhan, Sumaira Rashid, Nihar Ranjan Dash, Surabhi Gupta, Imteyaz Ahmad Khan, Nidhi Singh, Safoora Rashid, Vishwajeet Singh, Prasenjit Das, Ravindra Mohan Pandey
Jazyk: angličtina
Rok vydání: 2021
Předmět:
0301 basic medicine
Male
endocrine system diseases
Molecular biology
0302 clinical medicine
Cancer
Multidisciplinary
Gastroenterology
High-Throughput Nucleotide Sequencing
Middle Aged
Healthy Volunteers
Up-Regulation
Gene Expression Regulation
Neoplastic
medicine.anatomical_structure
030220 oncology & carcinogenesis
Biomarker (medicine)
Medicine
Female
Pancreas
Carcinoma
Pancreatic Ductal
Adult
Pancreatic ductal adenocarcinoma
Science
Down-Regulation
Article
Diagnosis
Differential
03 medical and health sciences
Young Adult
Downregulation and upregulation
Pancreatitis
Chronic
microRNA
medicine
Biomarkers
Tumor
Humans
Circulating MicroRNA
Aged
business.industry
Non invasive biomarkers
Liquid Biopsy
medicine.disease
digestive system diseases
Pancreatic Neoplasms
030104 developmental biology
ROC Curve
Case-Control Studies
Cancer research
Pancreatitis
Feasibility Studies
business
Biomarkers
Zdroj: Scientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
Scientific Reports
ISSN: 2045-2322
Popis: Early-stage diagnosis of pancreatic ductal adenocarcinoma (PDAC) is difficult due to non-specific symptoms. Circulating miRNAs in body fluids have been emerging as potential non-invasive biomarkers for diagnosis of many cancers. Thus, this study aimed to assess a panel of miRNAs for their ability to differentiate PDAC from chronic pancreatitis (CP), a benign inflammatory condition of the pancreas. Next-generation sequencing was performed to identify miRNAs present in 60 FFPE tissue samples (27 PDAC, 23 CP and 10 normal pancreatic tissues). Four up-regulated miRNAs (miR-215-5p, miR-122-5p, miR-192-5p, and miR-181a-2-3p) and four down-regulated miRNAs (miR-30b-5p, miR-216b-5p, miR-320b, and miR-214-5p) in PDAC compared to CP were selected based on next-generation sequencing results. The levels of these 8 differentially expressed miRNAs were measured by qRT-PCR in 125 serum samples (50 PDAC, 50 CP, and 25 healthy controls (HC)). The results showed significant upregulation of miR-215-5p, miR-122-5p, and miR-192-5p in PDAC serum samples. In contrast, levels of miR-30b-5p and miR-320b were significantly lower in PDAC as compared to CP and HC. ROC analysis showed that these 5 miRNAs can distinguish PDAC from both CP and HC. Hence, this panel can serve as a non-invasive biomarker for the early detection of PDAC.
Databáze: OpenAIRE
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