Mycobacterium abscessus induces a limited pattern of neutrophil activation that promotes pathogen survival
| Autor: | Scott D. Sagel, E. Michelle Nichols, Jerry A. Nick, Edward D. Chan, Kenneth C. Malcolm, Milene T. Saavedra, Jennifer L. Taylor-Cousar, Jennifer E. Kret, Lindsay J. Caverly, George M. Solomon, Paul R. Reynolds, Silvia M. Caceres, David P. Nichols, Donna L. Bratton, Stacey L. Martiniano |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Bacterial Diseases
Chemokine Necrosis Cystic Fibrosis Pulmonology Chronic Obstructive Pulmonary Diseases Gene Expression lcsh:Medicine Mycobacterium abscessus medicine.disease_cause Neutrophil Activation Autosomal Recessive Superoxides Molecular Cell Biology lcsh:Science Immune Response 0303 health sciences Multidisciplinary Innate Immunity 3. Good health Host-Pathogen Interaction Infectious Diseases Staphylococcus aureus Medicine Cellular Types medicine.symptom Research Article Immune Cells Phagocytosis Immunology Inflammation Biology Microbiology Mycobacterium Proinflammatory cytokine 03 medical and health sciences medicine Humans 030304 developmental biology Clinical Genetics Mycobacterium Infections 030306 microbiology Gene Expression Profiling lcsh:R Immunity Bacteriology biology.organism_classification bacterial infections and mycoses Emerging Infectious Diseases Biofilms Respiratory Infections biology.protein bacteria lcsh:Q Bacterial Biofilms |
| Zdroj: | PLoS ONE, Vol 8, Iss 2, p e57402 (2013) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | Mycobacterium abscessus is a rapidly growing mycobacterium increasingly detected in the neutrophil-rich environment of inflamed tissues, including the cystic fibrosis airway. Studies of the immune reaction to M. abscessus have focused primarily on macrophages and epithelial cells, but little is known regarding the neutrophil response despite the predominantly neutrophillic inflammation typical of these infections. In the current study, human neutrophils released less superoxide anion in response to M. abscessus than to Staphylococcus aureus, a pathogen that shares common sites of infection. Exposure to M. abscessus induced neutrophil-specific chemokine and proinflammatory cytokine genes. Although secretion of these protein products was confirmed, the quantity of cytokines released, and both the number and level of gene induction, was reduced compared to S. aureus. Neutrophils mediated killing of M. abscessus, but phagocytosis was reduced when compared to S. aureus, and extracellular DNA was detected in response to both bacteria, consistent with extracellular trap formation. In addition, M. abscessus did not alter cell death compared to unstimulated cells, while S. aureus enhanced necrosis and inhibited apoptosis. However, neutrophils augment M. abscessus biofilm formation. The response of neutrophils to M. abscessus suggests that the mycobacterium exploits neutrophil-rich settings to promote its survival and that the overall neutrophil response was reduced compared to S. aureus. These studies add to our understanding of M. abscessus virulence and suggest potential targets of therapy. |
| Databáze: | OpenAIRE |
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