Surface modification of solid-state nanopores for sticky-free translocation of single-stranded DNA
| Autor: | Dapeng Yu, Jiajun Wang, Zhipeng Tang, Qing Zhao, Bo Lu, Kaifu Luo |
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| Rok vydání: | 2014 |
| Předmět: |
Materials science
Surface Properties Movement DNA Single-Stranded Nanotechnology Polyethylene glycol Polyethylene Glycols Biomaterials chemistry.chemical_compound Nanopores Coated Materials Biocompatible General Materials Science Surface charge chemistry.chemical_classification Conductance Adhesiveness General Chemistry Polymer Sequence Analysis DNA Microarray Analysis Surface coating Nanopore chemistry Biophysics Surface modification DNA Biotechnology |
| Zdroj: | Small (Weinheim an der Bergstrasse, Germany). 10(21) |
| ISSN: | 1613-6829 |
| Popis: | Nanopore technology is one of the most promising approaches for fast and low-cost DNA sequencing application. Single-stranded DNA detection is primary objective in such device, while solid-state nanopores remain less explored than their biological counterparts due to bio-molecule clogging issue caused by surface interaction between DNA and nanopore wall. By surface coating a layer of polyethylene glycol (PEG), solid-state nanopore can achieve long lifetime for single-stranded DNA sticky-free translocation at pH 11.5. Associated with elimination of non-specific binding of molecule, PEG coated nanopore presents new surface characteristic as less hydrophility, lower 1/f noise, and passivated surface charge responsiveness on pH. Meanwhile, conductance blockage of single-stranded DNA is found to be deeper than double-stranded DNA, which can be well described by a string of blobs model for a quasi-equilibrium state polymer in constraint space. |
| Databáze: | OpenAIRE |
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