In-situ porcine corneal matrix hydrogel as ocular surface bandage
| Autor: | Tolou Shokuhfar, Ghasem Yazdanpanah, Khandaker N. Anwar, Sri Raghurama R Somala, Ritu Shah, Seungwon An, Ali R. Djalilian, Xiang Shen, Mark I. Rosenblatt, Meisam Omidi |
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| Rok vydání: | 2021 |
| Předmět: |
genetic structures
Swine Matrix (biology) Article Cornea Mice 03 medical and health sciences 0302 clinical medicine In vivo medicine Animals Humans 030304 developmental biology Corneal epithelium Wound Healing 0303 health sciences Decellularization Chemistry technology industry and agriculture Epithelium Corneal Hydrogels Bandages eye diseases In vitro Ophthalmology medicine.anatomical_structure 030221 ophthalmology & optometry sense organs Wound healing Bandage Corneal Injuries Biomedical engineering |
| Zdroj: | Ocul Surf |
| ISSN: | 1542-0124 |
| Popis: | PURPOSE: Bioactive substrates can be used therapeutically to enhance wound healing. Here, we evaluated the effect of an in-situ thermoresponsive hydrogel from decellularized porcine cornea ECM, COMatrix (COrnea Matrix), for application as an ocular surface bandage for corneal epithelial defects. METHODS: COMatrix hydrogel was fabricated from decellularized porcine corneas. The effects of COMatrix hydrogel on attachment and proliferation of human corneal epithelial cells (HCECs) were evaluated in vitro. The effect of COMatrix on the expressions of the inflammatory genes, IL-1β, TNF-α, and IL-6 was assessed by RT-PCR. The in-situ application and also repairing effects of COMatrix hydrogel as an ocular bandage was studied in a murine model of corneal epithelial wound. The eyes were examined by optical coherence tomography (OCT) and slit-lamp microscopy in vivo and by histology and immunofluorescence post-mortem. RESULTS: In vitro, COMatrix hydrogel significantly enhanced the attachment and proliferation of HCECs relative to control. HCECs exposed to COMatrix had less induced expression of TNF-α (P |
| Databáze: | OpenAIRE |
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